精子发生阻滞不育患者睾丸雌激素受体α的表达

Expression of estrogen receptor alpha in the testis of infertile men with spermatogenic arrest

目的:探讨雌激素受体α(ERα)在睾丸的表达与精子发生阻滞的关系。方法:用HE染色技术检查120例不育症患者睾丸活检标本,其中精子发生阻滞患者标本应用免疫组化法检查ERα的表达,并以10例健康人睾丸组织作为对照。结果:120例标本中符合精子发生阻滞病理诊断标准者有31例(占25.8%)。正常健康人睾丸组织中,ERα在睾丸支持细胞、类肌细胞和间质细胞的细胞核内表达,生精上皮细胞不表达;而在精子发生阻滞的睾丸组织中,ERα也在睾丸支持细胞、类肌细胞和间质细胞的细胞核内表达,但表达较弱,生精上皮细胞不表达。两者表达强度存在显著性差异(P<0.05)。结论:雄激素与雌激素通过各自的受体发挥协调作用而共同促进精子发生。精子发生阻滞可能与包括雄激素受体、ERα及热休克蛋白90在内的一系列复杂的细胞信号转导障碍有关。

Objective：To explore the association of spermatogenic arrest with the expression of estrogen receptor alpha（ERα） in human testes.Methods：We examined the testicular biopsy specimens of 120 infertile men by HE staining,detected the expression of ERα in the specimens of those with spermatogenic arrest by the two-step immunohistochemical method,and compared the results with those of 10 healthy men.Results：Of the 120 specimens from the infertile men,31（25.8%） met the diagnostic criteria of spermatogenic arrest.In the testis tissue of normal men,ERα expressed in Sertoli,myoid and Leydig cells,but not in spermatogenic cells,while in the testis tissues of those with spermatogenic arrest,ERα expressed lowly in Sertoli,myoid and Leydig cells,with statistically significant differences in immunostaining intensity between the two groups（P0.05）.Conclusion：Androgen receptor（AR） and ERα may play a coordinating role in facilitating spermatogenesis.Spermatogenic arrest may be related to a complex series of disorders in cell signal transduction involving AR,ERα and HSP90.