NOSTRIN在无精子症患者睾丸组织中的表达

Expression of NOSTRIN in the testis tissue of azoospermia patients

目的:通过测定无精子症患者睾丸组织中内皮型一氧化氮合酶运输介导物(NOSTRIN)和内皮型一氧化氮合酶(eNOS)的表达,探讨其与无精子症发病的相关性。方法:采用免疫组化法检测17例特发性无精子症患者(病例组)和10例正常男性(正常组)睾丸组织中NOSTRIN的定位和定量表达;RT-PCR检测睾丸组织中NOSTRINmRNA的表达;分光光度法测定睾丸组织中eNOS的活性;应用硝酸还原酶法测定睾丸组织上清液中NO代谢产物亚硝酸基/亚硝基(NO2-/NO3-)水平。结果:NOSTRIN在睾丸组织中表达于生精细胞、支持细胞以及血管内皮细胞,NOSTRIN在特发性无精子症患者睾丸中的表达水平显著低于正常男性;病例组患者睾丸组织中NOSTRINmRNA呈弱表达(0.312±0.076),明显低于正常组(0.793±0.082,P<0.01);病例组患者睾丸组织eNOS活性[(33.727±3.58)U/mg]与正常组[(17.69±3.84)U/mg]比较显著增高(P<0.01);病例组患者睾丸中NO2-/NO3-水平为[(48.56±8.49)μmol/L],与正常组[(25.37±9.61)μmol/L]比较显著升高(P<0.01);病例组睾丸组织中NOSTRIN表达水平与eNOS活性呈负相关(r=-0.57,P<0.01),与NO代谢产物NO2-/NO3-同样呈负相关(r=-0.61,P<0.01)。结论:无精子症患者睾丸组织中NOSTRIN表达水平降低,eNOS活性增强,NO-/NO-水平升高,这可能与无精子症的发生有着相关性。

Objective：To determine the expressions of endothelial nitric oxide synthase traffic inducer（NOSTRIN） and endothelial nitric oxide synthase（eNOS） in the testis tissue of azoospermia patients,and investigate their correlation with the pathogenesis of azoospermia.Methods：We detected the expressions of NOSTRIN and NOSTRIN mRNA by immunohistochemistry and RT-PCR respectively,determined the activity of eNOS by spectrophotometry,and measured the stable metabolic end product NO,NO2^-/NO3^-,by nitrate reductase assay in the testis tissues of 17 patients with idiopathic azoospermia（the azoospermia group） and 10 normal men（the normal group）.Results：NOSTRIN and NOSTRIN mRNA were expressed in the spermatogonia,sertoli cells,stromal cells and vascular endothelial cells,more lowly in the azoospermia than in the normal group（0.312 ± 0.076 versus 0.793 ± 0.082,P0.01）.The activity of eNOS was significantly increased in the idiopathic azoospermia patients（[33.727 ± 3.58] U/mg） compared with the normal men（[17.69 ± 3.84] U/mg）（P0.01）.The level of NO2^-/NO3^-was significantly higher in the azoospermia than in the normal group（[48.56 ± 8.49] μmol/L versus [25.37 ± 9.61] μmol/L,P0.01）.The expression of NOSTRIN showed a significant negative correlation with the activity of eNOS（r=-0.57,P0.01） as well as with the level of NO2^-/NO3^-（r=-0.61,P0.01） in the testis tissue of the idiopathic azoospermia patients.Conclusion：The expression of NOSTRIN is decreased,while the activity of eNOS and the level of NO2^-/NO3^-increased in the testis tissue of azoospermia patients,which may be associated with the pathogenesis of azoospermia.