溴隐亭致流产大鼠TGF-β1、IL-2、IL-4的表达变化及意义

The Expression Change and Its Significance of Transforming Growth Factor β1,IL-2 and IL-4 in Induced Abortion Rats by Bromocriptine

目的:研究转化生长因子-β1(TG9F-β1)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)在溴隐亭致流产大鼠黄体及血清的表达水平,探讨流产的发生机制。方法:将孕雌性wistar大鼠随机分为3组,正常孕组、注射溴隐亭组、注射溴隐亭+γ-干扰素(IFN-γ)抗体组,3组均于孕12天时,腹主动脉采血后心内灌注固定,酶联免疫吸附试验(ELISA)测定各组血清TGF-β1、IL-2、IL-4水平,免疫组化SP法检测TGF-β1、IL-2、IL-4在各组大鼠卵巢黄体的表达并进行图像分析,分别对上述结果进行单因素方差分析及t检验。结果:血清与卵巢黄体阳性细胞OD值的变化趋势基本一致,注射溴隐亭组,IL-2水平较正常孕组升高(P<0.05)、TGF-β1、IL-4水平较正常孕组降低(P<0.05);注射溴隐亭+IFN-γ抗体组,血清IL-2水平较注射溴隐亭组有所降低(P<0.05),但仍高于正常孕组(P<0.05),而TGF-β1、IL-4水平有所升高(P<0.05),基本达正常水平。结论:给孕鼠注射溴隐亭及IFN-γ抗体后,TGF-β1、IL-2、IL-4在血清及卵巢黄体的表达有一定改变,此改变可能与流产的发生有一定的关系。

Objective：To investigate the expression of transforming growth factor β1 （TGF-β1）, IL-2 and IL-4 in luteum body and serum in induced abortion rats by bromocriptine,and to explore the mechanism of abortion. Methods：Pregnant wistar rats were randomly divided into three groups： normal pregnancy group, bromocriptine injection group,bromocriptine and I FN-y antibody injection group. On the 12th day after pregnancy,the blood was collected from abdominal aorta followed by intracardiovascular perfusion fixation,serum level were checked by ELISA,while TGF-β1 ,IL-2 and IL-4 in luteum body by SP immunochemistry. Statistical analysis was done by univariate analysis and t-test. Results ：The changes of OD value in corpora luteum positive cells and serum level showed similar tendency. For the bromocriptine injection group, IL-2 level increased markedly than that in normal pregnancy（ P 〈0.05）, while transforming growth factor β1, IL-4 level decreased significantly （P 〈 0.05）. For bromocriptine and I FN-y antibody injection group, IL-2 level in serum decreased compared with that in bromocriptine group （ P 〈 0.05）, but still higher than that in normal pregnancy group （ P 〈0.05） ;while TGF-β1 and IL-4 level increased back to normal levle （ P 〈 0.05）. Conclusions：After injection of bromocriptine and IFN-y, antibody,the expressions of TGF-β1, IL-2 and IL-4 in serum and corpora luteum have some changes, and these changes probably associate with genesis of abortion.