酵母转录因子Gal4研究进展

Progress in the Study of Yeast Gal4 Transcription Factor

胁迫应答基因的转录激活是细胞应答胁迫作用的关键步骤。转录激活因子与启动子顺式作用元件结合是胁迫应答基因转录激活的关键环节。进化保守的Gal4是半乳糖代谢相关基因的转录激活因子。酵母Gal4通过其N端的DNA结合结构域识别并结合启动子UAS,通过其C端的激活结构域与转录因子作用,起始RNA聚合酶Ⅱ复合体的组装和转录。该过程不仅受转录调控因子Gal80和Gal3的调节,还与Gal4二聚体的形成有关。概述了酵母半乳糖代谢相关基因转录激活因子Gal4的研究进展。

The key step of cellular stress response is transcriptional activation of stress response gene including that transcription factors interact with cis-acting element.Gal4 protein is an evolutionary conserved transcription factor in the process of galactose metabolism.N-terminal DNA binding domain of yeast Gal4 protein binds to the Upstream Activating Sequence and C-terminal activation domain interacts with general transcriptional factors,recruit RNA polymerase Ⅱ to TATA-box.This process is regulated not only by regulating factors Gal80 and Gal3,but also relies on Gal4 dimerization.The role of the yeast Gal4 plays on the transcriptional activation of galactose metabolism genes is illustrated.