摘要
随着对绿色荧光蛋白(green fluorescent protein,GFP)研究的不断深入,人们对其结构、荧光产生机理等已有较为全面的认识。近年来利用GFP及其它荧光蛋白(FPs)发展了诸如荧光互补技术(FC)、荧光共振能量转移技术(FRET)和超分辨成像(super-resolution imaging)等一系列新技术,极大地促进了生物学、医药科学的研究。主要介绍了荧光蛋白的结构,荧光产生的机理,不同类型的荧光蛋白和基于荧光蛋白产生的新技术等方面的最新研究进展。
The structure and fluorescence mechanism of green fluorescent proteins(GFP) have been well understood after decades of study.Based on these knowledge,scientists discovered and developed a range of novel fluorescent proteins(FPs) with a wide emission spectrum covering from 424655 nm.With the colorful palette of FPs,new technologies such as fluorescence complementation(FC) fluorescence resonance energy transfer(FRET) and super-resolution imaging have been developed and act as powerful tools in biological and medical studies.The latest progress about the structure,chromophore maturation and fluorescence mechanism of GFP,as well as the extensive FP families and the new technologies based on them were provided.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2011年第1期96-102,共7页
China Biotechnology
关键词
绿色荧光蛋白(GFP)
生色团
荧光产生机理
荧光蛋白的应用
Green; fluorescent; proteins(GFP); Chromophore; Fluorescence; mechanism; Application; of; fluorescence; proteins;