CD-TK双自杀基因联合染料木黄酮治疗前列腺癌的实验研究

Experimental Study on Combined Therapy of Double Suicide Gene and Genistein in Prostate Carcinoma

目的:研究CD-TK双自杀基因联合染料木黄酮对鼠前列腺癌的体内治疗作用。方法:取鼠前列腺癌细胞RM-1接种于鼠C57BL/6背部皮下建立移植瘤模型。将荷瘤鼠随机分为对照组、术黄酮组、TK/DC治疗组及TK/CD+染料术黄酮联合治疗组进行相应处理,实验结束后比较各组肿瘤体积、全部肿瘤标本行常规病理检验及bcl-2免疫组化检测。结果:对照组肿瘤体积为(1008.73±126.73)mm^3.染料木黄酮组肿瘤体积为0359.06±53.17)mm^3,双自杀基因组肿瘤体积为(222.60±26.79)mm^3.两者联合时肿瘤体积为(25.31±9.24)mm^3。双自杀基因组鼠肿瘤体积比染料木黄酮组小(P<0.01).两者联合时肿瘤体积最小(P=0.00。病理切片中显示对照组几乎未见细胞坏死.而联合用药组肿瘤大片坏死;免疫组化检测肿瘤标本中bcl-2含量显示对照组显强阳性.而联合治疗组仅见微弱阳性。结论:联用染料木黄酮能明显增强CD-TK双自杀基因系统对前列腺癌的治疗作用。

Objective： To investigate the effects of combined therapy of CEVTK double suicide gene and genistein in mouse prostate carcinoma in vivo. Methods： RM-1 cells,a mouse prostate carcinoma cell line,were im- planted into the subcutaneous tissue of the back of C57BL/6 mice to establish an animal model of PCa. Then all the mouse with tumors were divided into 4 groups as control group, TK/CD group, genistein group and combined therapy group and treated accordingly. In the end, the tumors volume Were measured to evaluate therapeutic effects,routine pathological investigation and immunohistoehemical staining of bcl-2 were taken in all the samples. Results： The mean tumor volume was （1 008.73±126.73） mm3 in control group, （359.06± 53.17） mm3 in genistein group, （222. 60±26.79） mma in TK/CD group but only （25.31 ± 9.24） mm3 in combined therapy group. Inhibition of tumor growth and extensive necrosis were observed most significantly in combined therapy group. The expression of hcl-2 was generally high in the control group but low in the treated ground,especially in the combined therapy group. Conclusions： CD-TK double suicide combined with genistein treatment may enhance the therapeutic effects in prostate carcinoma