黄河流域棉区转Bt基因棉种植对根际土壤微生物数量及细菌多样性的影响

Effect of plantation of transgenic Bt cotton on the amount of rhizospheric soil microorganism and bacterial diversity in the cotton region of Yellow River basin

采用传统培养与PCR-DGGE相结合的方法研究了黄河流域棉区4个省份种植的转Bt基因棉在4个生长时期(播种后第30天、第60天、第90天和第120天)的根际土壤微生物数量及细菌多样性.结果表明:同一省份同一生长时期转Bt基因棉根际土壤微生物数量与常规棉相比均无显著差异,其数量主要受不同生长时期影响,而不同省份间主要受地域条件的影响.4个省份转Bt基因棉根际土壤细菌多样性较丰富,同一省份同一生长时期内转Bt基因棉与常规棉根际土壤细菌多样性指数、均匀度和丰富度均无显著差异.不同省份间细菌多样性主要因地域条件而有所不同,但差异较小.

Traditional culture-dependent method and PCR-DGGE were adopted to investigate the amount of microorganism and bacterial diversity in rhizospheric soil of transgenic Bt cotton in four provinces of Yellow River basin at four growth stages,i.e.,30,60,90,and 120 days after sowing. In the same province and at the same growth stage,no significant difference was observed in the amount of microorganism in rhizospheric soils of transgenic and non-transgenic Bt cottons. Within the same province the amount of microorganism was mainly affected by growth stage; while in different provinces,it was greatly affected by regional conditions. In the four provinces,the bacterial diversity in rhizospheric soil of transgenic Bt cotton was abundant; and in the same province and at the same growth stage,there were no significant differences in the Shannon index,evenness,and richness of bacteria in rhizospheric soils of transgenic and non-transgenic Bt cottons. In different provinces,the bacterial diversity in rhizospheric soils was dependent on regional conditions,but the difference was rather small.