色素上皮衍生因子抑制高糖诱导的人肾小球系膜细胞转化生长因子β1、纤连蛋白和Ⅳ型胶原的表达

Pigment epithelium-derived factor inhibits high glucose-stimulated transforming growth factor β1,fibronectin, and collagen Ⅳ expressions in human mesangial cells

随着葡萄糖浓度的增加,人肾小球系膜细胞转化生长因子β1（TGF-β1）、纤连蛋白（FN）和Ⅳ型胶原（ColⅣ）的表达显著增加（P＜0.01）.在30 mmol/L的高糖条件下,40～160 nmol/L的色素上皮衍生因子（PEDF）显著抑制TGF-β1表达,呈剂量依赖性（P＜0.01）;5～40 nmol/L的PEDF也剂量依赖性地抑制FN和ColⅣ的表达（P＜0.01）,提示PEDF可能通过其抗纤维化的作用延缓糖尿病肾病的进程.

The expressions of transforming growth factor β1 （TGF-β1）, fibronectin （FN）, and collagen Ⅳ in human mesangial cells were augmented with increased concentrations of glucose.Pigment epithelium-derived factor （PEDF） at concentrations of 40-160 nmol/L significantly inhibited TGF-β1 expression in a dose-dependent manner（P〈0.01）.PEDF at concentrations of 5-40 nmol/L significantly decreased FN and collagen Ⅳ expressions in a dose-dependent manner（P〈0.01） ,suggesting that PEDF may play a salutary role in diabetic nephropathy by its antifibrogenic activity.