基质细胞衍生因子-1α在皮质发育畸形大鼠海马中的表达

The expression of SDF-1α in the malformation of cortical development model rats

目的了解基质细胞衍生因子-1α(SDF-1α)在皮质发育畸形(MCD)大鼠海马中的表达,探讨其在皮质发育畸形异常局部神经回路中的作用。方法通过腹部X射线照射孕16dSD大鼠构建子代MCD模型,对30、60d对照组与实验组子鼠(P30、P60)行HE、Timm染色以检测其组织学改变,免疫组化、RT-PCR及Western-Blot检测SDF-1α的表达情况。结果①Timm染色示模型鼠海马出现苔藓纤维出芽;②免疫组化示SDF-1α在模型组海马CA1区异位神经元有表达;③RT-PCR和Western-Blot结果显示模型组P30、P60较同期的对照组表达降低。结论 SDF-1α的下调可能参与皮质发育畸形海马中异常神经回路的形成。

Objective To get a message of the expression about stromal cell-derived factor-1a （SDF-1a）in the Malformations of cortical development （MCD）in rats model and investigate the effects in forming abnormal neural circuit in the MCD. Methods Sprague-Dawley pregnant rats exposed to X-irradiation on embryonic 16 （E16）days can induce MCD in the offspring. We observed the histological changes of the brain with HE staining and Timm staining,and the expression of SDF-1a with immunohistochemistry, RT-PCR and Western-Blot in the pups at postnatal 30 days （P30）and postnatal 60 days （P60）. Remalts ①Timm staining demonstrated mossy fiber sprouting was formed in the MCD model; ② Immunohistochemical results showed that expression of SDF-1a appeared in the CA1 ectopic neurons of hippocampus; ③From the results of RT-PCR and Western-Blot, we got a reduced expression of SDF-1α at P30 and P60 in the model group contrast to control group at the same period. Conclusion the downregulation of SDF-1a may play a role in forming abnormal neural circuit in hippocampus of MCD.