摘要
目的评估PDLLA/PLA-PEG-PLA在骨组织工程中的应用前景。方法在4只恒河猴双侧顶骨24个10 mm颅骨去骨膜全层骨缺损中分别植入4种筛选出的支架/成骨细胞复合体,术后6、12周采用X线、组织学及扫描电镜观察评价骨缺损修复情况。结果大体观察,都未引起明显的全身反应,局部切口愈合良好。X线、组织学及扫描电镜均证实人工骨在术后6周、12周成骨能力明显高于空白对照组,也高于单纯注射成骨细胞对照组,而改性的PDLLA/PLA-PEG-PLA、PDLLA/β-TCP及PDLLA/BG成骨能力优于单纯PDLLA,X线评分证实PDLLA/PLA-PEG-PLA成骨能力最强,12周时成骨量达缺损区70%。结论 4种筛选的支架与成骨细胞复合构建组织工程人工骨具有较强的体内成骨效能,PDLLA/PLA-PEG-PLA在骨组织工程中有很好的应用前景。
Objective To evaluate the prospect of PDLLA/PLA-PEG-PLA for bone tissue engineering.Met.hods Each of 24 cranial defects with 10 mm diameter and complete resection of periosteum of adult rhesus monkeys was implanted with four kinds of composite scaffolds seeding osteoblasts with good cell biocompatibility in cell culture model. The specimens were harvested in 6, 12 weeks. The samples were examined by X-ray, histology and scanning electronic microscope （SEM） to evaluate the effect of defect repair. Results All of the experimental animals survived from the operation and the incisions healed without special treatment. The results obtained from radiographic bone formation,histologic analysis and SEM indicated the PDLLA, PDLLA/BG, PDLLA/β-TCP and PDLLA/PLA-PEG-PLA cell-polymers stimulated more bone formation than control group and osteoblasts injection group. And PDLLA/BG, PDLLA/β-TCP and PDLLA/PLA-PEG-PLA cell-polymers have more bone formation than PDLLA cell-polymers. The scores of X-ray examination demonstrated a maximum bone formation of 70% of defects with PDLLA/PLA-PEG-PLA cell-polymers. Conclusion Four kinds cell-polymers of PDLLA, PDLLA/BG, PDLLA/β-TCP and PDLLA/PLAPEG-PLA can be used for bone tissue engineering to improve the bone formation in vivo. PDLLA/PLAPEG-PLA was a new valuable scaffold and showed a applied prospect for bone tissue engineering.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2011年第1期77-82,共6页
Chinese Journal of Clinical Anatomy
基金
广东省科技计划(2010B080701019)
