一种新型大鼠椎间融合模型的建立及评价

A novel kind of spinal fusion model-rats interbody fusion

目的建立一种新型的大鼠脊柱融合模型,并证实不同融合骨量对融合的影响。方法雄性SD大鼠60只,随机分成3组(n=20),制备椎间融合模型,取双侧髂骨制成颗粒骨,A组0.3 cm3颗粒骨植入待融合节段,B组0.2 cm3颗粒骨植入待融合节段,C组空白对照组,融合节段选择第1尾椎。3、6周后进行手触检查、X线评分,6周后组织学观察。结果 3周时手触检查:A组融合率为10%、B组、C组无一例出现融合;6周:A组融合率为40%,B组为40%,C组未融合。X线评分系统,术后3周:A组(2.567±0.876)分,B组为(2.467±0.878)分,C组为(0.233±0.316)分;术后6周:A组为(3.233±1.218)分,B组为(3.267±1.235)分,C组为(0.467±0.322)分。组织学观察可见实验组已融合区有大量新生骨形成,C组可见纤维组织分布占大多数。结论本实验成功验证了一种新型大鼠脊柱融合模型。

Objective To establish a novel kind of fusion model, and provide the influence of fusion by different bone mass in rats interbody fusion model. Methods Sixty male Sprague-Dawley rats underwent interbody fusion with bilateral iliac bone and 1-tailbone autograft into intertailbone. 0.3 cm3 autograft bone was transplanted into the fusion segments of group A and 0.2 cm3 autograft bone was transplanted into that of group B. In contrast, control group C had no bone placed. Fusion segments were removed and analyzed with manual bending and radiography at 3 and 6 weeks, respectively. Histologic analysis was performed at 6 weeks. Half were sacrifced at 3 weeks; the others were sacrifced at 6 weeks. Results At 3 weeks, 10%（1/10） of group A rats fused, 0%（0/10） of group B rats fused and 0%（0/10） of group C rats fused by manual palpation. At 6 weeks, 40%（4/10） of group A rats fused, 40%（4/10） of group B rats fused and 0%（0/10） of group C rats fused by manual palpation. By radiographic scoring system （＂0＂no bone formation,＂5＂full fusion）, the scores of group A,group B and group C were evaluated as （2.567±0.876）, （2.467±0.878）and （0.233±0.316）at 3 weeks after operation, in contrast with （3.233±1.218）, （3.267±1.235）and （0.467±0.322）at 6 weeks after operation, respectively. From our gross observation, bone formation with interconnected bone trabecula, which existed in the fusion area of the experimental group, was verified by HE and VG staining at 6 weeks. The neogenesis of the osteoblast and the active bone remodeling with the irregular bone cement lines were seen at the higher magnification. However, there were no existence of bone fusion area in the control group, the fibers tissue is distributed instead. Conclusions Our research created a new animal model for spine fusion and verified the effect of quantity of bone autograft on fusion.