载黄芪多糖羟基磷灰石的制备及生物学性能评价

Preparation and Biological Properties of HA Containing Astragalus Polysaccharides

室温湿法合成载不同浓度黄芪多糖(APS)的羟基磷灰石(HA/APS),通过X射线衍射(XRD)表征APS对HA晶体结构、结晶度及晶粒尺寸的影响;用透射电子显微镜(TEM)表征HA和HA/APS的晶体形貌,用激光粒度仪和比表面积仪分别检测HA的粒度及比表面积;体外培养MC3T3-E1成骨细胞,进行Alamar Blue、噻唑蓝(MTT)及碱性磷酸酶(ALP)检测,光镜观察细胞形貌,研究APS对成骨细胞作用的有效浓度范围及HA/APS对成骨细胞活性及分化的影响.结果表明:所载APS对HA晶体结构、结晶度和晶粒尺寸没有明显影响,晶体形貌没有变化,HA平均粒径为1.17μm,比表面积为132.194 m^2/g;APS对成骨细胞的作用呈剂量和时间依赖关系,80～200μg/mL促进细胞活性及ALP表达,HA/APS增强细胞活性,细胞形貌完整.因此,0.5 g HA载入100～250μg APS时明显促进成骨细胞活性,HA/APS具有作为临床骨缺损填充材料的潜能.

Hydroxyapatite（HA） powders containing different concentrations of astragalus polysaccharides（APS） were synthesized by a wet chemical method.The effect of APS on the crystal structure,crystallinity and crystal size of HA was characterized by XRD,the crystal morphologies of HA and HA/APS were characterized by TEM,the particle size and specific surface area of HA were detected by laser partical size distribution analyzer,automated surface area and pore size analyzer,respectively.Furthermore,the effective concentration of APS on MC3T3-E1 osteoblasts,HA/APS on osteoblastic activity and differentiation was evaluated by Alamar blue,MTT and alkaline phosphatase assay.Cell morphologe was observed by light microscope.The results showed that the crystal structure, crystallinity and crystal size of HA were not obviously effected by the presence of APS.Crystal morphologies of HA/APS was almost the same compared with HA,the average particle size of HA was 1.17μm and specific surface area was 132.194 m^2/g.The effect of APS on osteoblasts was time and dose-dependent,APS concentrations within 80-200μg/mL enhanced osteoblastic activity and ALP expression,HA/APS enhanced osteoblastic activity and the osteoblasts had integrated morphology.It can be concluded that 0.5 g of HA containing 100-250μm APS can promote osteoblastic activity.HA/APS has the promising potential as bone defect filling material for clinical applications.