成纤维细胞和血小板在多壁碳纳米管上粘附性

Study of Fibroblast and Platelet Adhesion on Multi-wall Carbon Nanotubes

使用化学气相沉积（CVD）的方法在碳纸基底上制备了多壁碳纳米管（MWCNTs）,并研究了L929小鼠成纤维细胞在多壁碳纳米管和碳纸对照组上的粘附及增殖等生长行为,以及各种血液蛋白吸附于这两种材料表面后对人皮肤成纤维细胞粘附产生的影响,同时对比了这两种材料的血小板粘附情况.结果表明：种植在多壁碳纳米管上的成纤维细胞生长明显比碳纸上的旺盛,细胞浓度从第1天的12.5×10^5/mL明显增加到第7天的4.1×10^5/mL,多壁碳纳米管对细胞无毒性反应.预吸附白蛋白、纤维蛋白原、免疫球蛋白对细胞粘附量有促进作用,并且多壁碳纳米管的血小板粘附率低于碳纸.这些结果证明多壁碳纳米管具有良好的组织相容性和一定的血液相容性.

Multi-walled nanotubes（MWCNTs） were grown on carbon papers by chemical vapor deposition（CVD）. Adhesion and proliferation of L929 mouse fibroblasts and platelets on MWCNTs and carbon papers were investigated. The influence of different blood protein adsorption on the human skin fibroblasts growth was also studied. The results showed that mouse fibroblasts implanted on MWCNTs tended to grow more prolifically than those on carbon papers.The cell concentration observed on MWCNTs increased from 1.25×10^5/mL（cultured for 1 d） to 4.1×10^5/mL（culture for 7 d）.No toxicity reaction was observed during the culturing period.By employing the adsorption assay,it was found albumin,fibrinogen,and immunoglobulin G adhered on materials could promote the adhesion and growth of the human skin fibroblasts.Furthermore,platelet adhesion rate on MWCNTs is lower than that on the carbon papers.These results indicate that MWCNTs have better tissue compatibility and blood compatibility.