在小鼠帕金森病模型中观察丙戊酸钠对多巴胺神经元及GDNF表达的影响(英文)

Effects of valproate on dopaminergic neurons and expressions of GDNF in Parkinson's disease mice

目的:通过建立小鼠帕金森病模型,观察丙戊酸钠(valproate,VPA)对中脑黑质多巴胺神经元及胶质源性神经营养因子(glial-derived neurotrophic factor,GDNF)表达的影响,以期探索丙戊酸钠的神经保护作用机制。方法:采用免疫组织化学方法检测小鼠中脑黑质多巴胺神经元;原位杂交方法检测纹状体、海马、顶叶皮质GDNF的表达。结果:GDNF在模型组和VPA组表达明显增加。结论:VPA可能通过上调GDNF的表达而起到神经保护作用。

Objective：To investigate the expression of glial-derived neurotrophic factor（GDNF） in mice with Parkinson＇s disease,and to study the neuroprotective effects of valproate（VPA）.Methods：The animals were randomly divided into four groups,including normal saline（NS）＋NS group,NS＋MPTP group,VPA ＋MPTP group and VPA＋NS group,with 6 mice in each group.Tyrosine hydroxylase（TH） in the substantia nigra pars compacta（SNc） was measured by immunohistochemistry.The expression of GDNF in striatum,hippocampus and cerebral cortex was observed by in situ hybridization.The A values of positive neurons of GDNF was scaned and analyzed statistically.Results：The expression of GDNF was significantly increased in normal saline（NS）＋MPTP group,VPA ＋MPTP group and VPA＋NS group compared to NS＋NS group.Conclusion：Protective effect of VPA against Parkinson＇s disease may be associated with increased expression of GDNF.