二苯乙烯苷通过抑制ROS减轻6-OHDA诱导的PC12细胞凋亡

TSG attenuated 6-OHDA-induced apoptosis in PC12 cells by the inhibition of ROS

目的:探讨2,3,5,4’-四羟基二苯乙烯-2-o-β-D-葡萄糖苷(2,3,5,4’-tetrahydroxystibene-2-o-β-D-gluco-side,TSG)对6-羟基多巴胺(6-hydroxydopamine,6-OHDA)诱导的PC12细胞凋亡的影响及其可能的机制。方法:4-甲基偶氮唑蓝(MTT)检测PC12细胞活性;流式细胞仪(FCM)检测PC12细胞凋亡百分率;活性氧(reactive oxy-gen species,ROS)检测试剂盒测定细胞内ROS水平。结果:与6-OHDA处理组(52.6±1.3)%比较,10,20,50μmol/L TSG预处理后,6-OHDA对PC12细胞的毒性明显减轻,使细胞活力分别上升为(66.6±3.2)%(P<0.01),(73.0±1.6)%(P<0.01),(83.9±1.5)%(P<0.01)。流式细胞仪检测显示正常组,6-OHDA组,TSG(10,20,50μmol/L)预处理组,细胞凋亡百分率分别为2.4%,49.9%,25.6%,14.1%,8.5%。TSG(10,20,50μmol/L)预处理后,对比6-OHDA处理组(261.4±8.4)%,PC12细胞内产生的ROS水平分别降低为(255.6±6.8)%,(229.0±9.1)%(P<0.05),(149.2±9.4)%(P<0.01)。结论:TSG对6-OHDA诱导的PC12细胞凋亡具有保护作用,其机制可能与TSG抑制ROS有关。

Objective：To explore the protective effect of 2,3,5,4＇-tetrahydroxystibene-2-o-β-D-glucoside,TSG） on 6-hydroxydopamine（6-OHDA）-induced apoptosis in PC12 cells and its possible mechanism.Methods：The cell viability was measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyhetrazolium bromide（MTT） assay,the apoptotic percentage of PC12 cells was detected by flow cytometry（FCM）and the level of reactive oxygen species（ROS） production in PC1 2 cells was measured by reactive oxygen species assay kit.Results：Compared with 6-OHDA treated cells（52.6±1.3）%,cell viability was increased to（66.6±3.2）%（P0.01）,（73.0±1.6）%（P0.01）,（83.9±1.5）%（P0.01） respectively after exposure with 10,20 and 50 μmol/L TSG.The apoptotic percentage of PC12 cells are 2.4%,49.9%,25.6%,14.1% and 8.5% respectively by FCM.In addition,the level of ROS production in PC12 cells markedly decreased to（255.6±6.8）%,（229.0 ±9.1）%（P0.05）,（149.2±9.4）%（P0.01） respectively after After pretreatment with 10,20 and 50 μmol/L TSG,compared with the 6-OHDA group（261.4±8.4）%.Conlusions：TSG has protective effect on 6-OHDA-induced apoptosis of PC12 cells,which might be related to the inhibition of TSG on ROS.