珠子参水提物预处理对小鼠脑缺血再灌注损伤的影响

Effect of pretreatment with water extract from Rhizoma Panacis majoris on cerebral ischemia-reperfusion injury in mice

目的研究珠子参水提物预处理对小鼠局灶性脑缺血再灌注损伤的保护作用。方法雄性5周龄昆明小鼠170只,采用完全随机设计分组法分为6组:假手术组(n=20)、模型组(n=30)、尼莫地平组(2 mg/kg,n=30)和珠子参水提物低、中、高剂量组(2.5、5.0、10.0 g/kg,n=30)。除假手术组和模型组灌胃给予蒸馏水20 ml/kg外,其余各组灌胃给予相应剂量的药物。以上各组给药7 d后制作大鼠大脑中动脉闭塞模型,假手术组切开缝合不进行动脉阻塞,在缺血2 h再灌注24 h后进行行为学检测,记录各组小鼠存活率。取大脑并用2,3,5-三苯基氯化四氮唑染色后测定梗死面积和脑含水量;制作脑匀浆测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)、黄嘌呤氧化酶(XOD)酶活性和丙二醛(MDA)含量。结果珠子参水提物中、高剂量组的成活率分别为77.8%(21/27)和82.1%(23/28),与模型组[51.9%(14/27)]比较具有统计学差异(P<0.05);珠子参水提物高剂量组的神经症状评分(1.77±0.38)、斜板角度(63.6±5.5)°优于模型组[神经症状评分(2.21±0.43),斜板角度(55.1±7.5)°](P<0.05,P<0.01),与尼莫地平效果接近;与模型组比较,珠子参水提物中、高剂量组能显著降低脑梗死面积和脑含水量(P<0.05,P<0.01),并能明显增加SOD、GSH-PX、CAT酶活性(P<0.01),降低XOD酶活性和MDA含量(P<0.01);高剂量组在改善GSH-PX、SOD和CAT酶及XOD酶活性方面优于尼莫地平组。结论珠子参水提物预处理对小鼠局灶性脑缺血再灌注损伤具有明显的保护作用。

Objective To study the protective effect of water extract from Rhizoma Panacis majoris on cerebral ischemia-reperfusion（I/R） injury in mice.Methods Mice were divided into low dose（2.5 g/kg）,medium dose（5.0 g/kg） and high dose（10.0 g/kg） Rhizoma Panacis majoris water extractive of Rhizoma Panacis majoris groups,nimodipine group and sham group respectively,after pretreating homologous drugs for 7 days.The mouse model of middle cerebral artery occlusion was induced,and neurological symptom scoring and inclined board testing were performed within 24 h after I/R.The survival rate,infarct volume and neuron density of mice were recorded.The levels of superoxide dismutase（SOD）,glutathione peroxidase（GSH-PX）,catalase（CAT）,malondialdehyde（MDA）,xanthine oxidase（XOD） were measured.Results The survival rate was higher in medium and high dose groups than in model group（77.8% and 82.1% vs 51.9%,P0.05）.The neurological symptom score and inclined board degree were greater in high dose group than in model group（1.77±0.38 and 63.6±5.5 vs 2.21±0.43 and 55.1±7.5,P0.05）,which were similar to those in nimodipine group.The infarct size and water content were significantly lower in medium and high dose groups than in model group（P0.05）,which significantly increased the activity of SOD,GGSH-PX and CAT while decreased the activity of XOD and MDA level（P0.05）.The improvement in activity of SOD,GGSH-PX and CAT was better in high dose group than in nimodipine group.Conclusion Pretreatment of water extract from Panacis majoris rhizoma can significantly protect mice against cerebral I/R injury.