夏氏疟原虫感染早期易感和抵抗小鼠树突状细胞亚群和表型的变化特点

Dendritic cell subpopulation and phenotype variation characteristics in susceptible and resistant mice during the early phase of Plasmodium chabaudi chabaudi infection

目的探讨夏氏疟原虫（Plasmodium chabaudi chabaudi AS）感染早期,树突状细胞（Dendritic cells,DCs）亚群和表型的变化特点。方法感染易感的DBA/2和抵抗的BALB/c小鼠,制备感染前和感染后第3、5、8d小鼠脾细胞悬液,采用流式细胞分析技术检测2种小鼠脾细胞悬液中髓样DCs和浆样DCs的数量以及表面表达MHC Ⅱ类分子和CD80分子的DCs的百分含量。结果 DBA/2小鼠和BALB/c小鼠分别呈现以髓样DCs和浆样DCs为主的增殖模式。2种小鼠表达MHCⅡ类分子和CD80分子的DCs数量在感染后3~5d明显升高,并于感染后第8d达到最高水平,然而感染后第8d,BALB/c小鼠表达MHCⅡ类分子和CD80分子的DCs的数量明显低于DBA/2小鼠。结论感染早期,DBA/2和BALB/c小鼠在DCs亚群的增殖模式、成熟表型特征性分子的表达水平上存在显著差异。

To investigate the dendritic cells（DCs） subpopulation and phenotype characteristics during early infection of Plasmodium chabaudi chabaudi（AS）.The susceptible DBA/2 mice and the resistant BALB/c mice were infected by intraperitoneal injection of 1 106 P.c.chabaudi parasitized erythrocytes.Spleen cell suspension was prepared from the mice sacrificed on days 0,3,5,8 post infection（p.i.）.The percentages of myeloid DCs,plasmacytoid DCs,as well as surface molecule MHCII or CD80 expressed on spleen DCs were determined by flow cytometry.The results indicated that DBA/2 mice and BALB/c mice respectively presented myeloid DCs and plasmacytoid DCs dominant proliferation pattern.The expression of MHCⅡand CD80 were significantly increased on days 3-5 p.i.and reached its peak on day 8 p.i.for BALB/c and DBA/2 mice.However,their expression levels in BALB/c mice were significantly lower than that in DBA/2 mice on day 8 p.i..These results indicated that there are significant differences in subpopulation proliferation model and the surface marker expression for DCs in BALB/c and DBA/2 mice during the early stages of P.c.chabaudi infection.