CXCR4在喉鳞状细胞癌中的表达及与微血管形成的相关性研究

The expression and relationship of CXCR4 and microvessel density in laryngeal squamous cell carcinoma

目的:检测CXCR4的mRNA及其蛋白在喉鳞状细胞癌中的表达情况,分析CXCR4的表达与喉鳞状细胞癌的临床病理特征的关系,探讨CXCR4在喉鳞状细胞癌的发生、发展、转移中的作用;分析CXCR4与肿瘤微血管形成的关系,探讨CXCR4在喉鳞状细胞癌的肿瘤血管形成中的作用。方法:应用RT-PCR及免疫组织化学技术,分别对42例新鲜喉癌组织及远离肿瘤组织的正常喉黏膜的CXCR4的mRNA和蛋白表达情况进行测定,并与肿瘤患者的临床病理特征(性别、年龄、病变部位、T分期、病理分级、颈淋巴结转移)相比较;对肿瘤再生血管用免疫组织化学方法对抗Ⅷ因子相关抗原抗体进行测定。应用Image-pro-plus软件进行肿瘤细胞光吸收度的定量分析。结果:CXCR4在喉癌组织中呈过度表达,而自身正常喉黏膜弱表达或不表达,在2种组织中差异有统计学意义(P<0.01);在肿瘤组织中,CXCR4的表达与患者的性别、年龄、病变部位、T分期无关(P>0.05);而与病理分级相关(P<0.01),与是否有颈淋巴结转移相关(P<0.01)。蛋白表达水平情况与基因表达水平相一致。肿瘤再生微血管数与喉鳞状细胞癌的mRNA及蛋白表达水平呈显著正相关(R=0.996 79,P<0.01)。结论:CXCR4在喉鳞状细胞癌组织中高表达,并在喉鳞状细胞癌的浸润和转移中起重要作用,且与肿瘤微血管形成有关。

Objective：To investigate the expression of chemokine receptor 4（CXCR4） in laryngeal squamous carcinoma and its correlation with clinicopathology character and the building of microvessel density（MVD）.To evaluate its role in the carcinogenesis and progression in laryngeal squamous carcinoma.Method：The expression of CXCR4 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 42 patients were examined by RT-PCR,immunohistochemical staining and Image-pro-plus software.The numbers of regeneration blood vessels in the laryngeal squamous carcinoma was counted by antibody against factor Ⅷ associated antigen and immunohistochemical analysis.Result：The positive expression rate of CXCR4 in tumor samples was significantly higher than that in normal ones（P0.01）.In tumor samples,the expression of CXCR4 were not associated with age,sex,tumor site and T stage（P0.05）,while it were higher in tumors of grade Ⅲ,Ⅳ than in grade Ⅰ,Ⅱof pathology classification（P0.01）.The expression of CXCR4 were signifycantly higher in tumors with cervical lymph node metastasis than that in tumor without cervical lymph node metastasis（P0.01）.The expression of CXCR4 protein and CXCR4 mRNA were at the same level.The expression level of CXCR4 in the laryngeal squamous carcinoma tissue was positively correlated with vascularization.Conclusion：The higher expression of CXCR4 may play a key role in the invasion and metastasis of laryngeal squamous carcinoma,and were correlated with micro-vascularization.