颗粒酶B和CD8在不明原因复发性流产蜕膜组织中的表达

Expressions of granzyme B and CD8 in decidual tissues of cases with unexplained recurrent spontaneous abortion

目的:比较不明原因复发性流产(URSA)与正常早孕蜕膜CD8和颗粒酶B的表达,从蛋白水平探讨不明原因复发性流产的发病机制。方法:用免疫组织化学方法测定两组蜕膜CD8和颗粒酶B蛋白表达,采用双盲法随机取5个视野,400倍下观察蜕膜组织处阳性细胞,计数后取其平均数进行半定量分析。结果:与正常早孕相比,URSA组蜕膜组织处颗粒酶B表达明显升高(P<0.01);CD8的表达两组比较无明显差异(P>0.05)。结论:早孕期蜕膜组织颗粒酶B蛋白表达异常是URSA的机制之一;颗粒酶B死亡途径可能是早期诱导URSA发生的重要因素。

Objective：To compare the expressions of granzyme B and CD8 in decidual tissues of cases with unexplained recurrent spontaneous abortion（URSA） and normal early pregnant women,explore the mechanism of URSA according to protein level.Methods：Immunohistochemical method was used to detect the expressions of granzyme B and CD8 in decidual tissues of cases with URSA,5 visual fields were selected randomly by double-blind method,positive cells in decidual tissues were observed under microscope（400×）,mean was obtained after counting for semiquantitative analysis.Results：Compared with normal early pregnancy group,the expression level of granzyme B in decidual tissues of URSA group increased significantly（P0.01）;but there was no significant difference in expression level of CD8 between the two groups（P0.05）.Conclusion：The abnormal expression of granzyme B protein in decidual tissues of URSA group is one of the mechanisms of URSA;the pathway of cell death induced by granzyme B may be an important factor of occurrence of URSA.