制半夏煎剂减少兔脑梗死再灌注出血转化的研究

Study of Pinellia decoction on decreasing hemorrhagic transformation in rabbits with cerebral infarction and reperfusion

目的研究制半夏煎剂对兔脑梗死再灌注出血转化的作用。方法新西兰兔106只,分为实验组和对照组,实验前2 h,实验组以制半夏水煎浓缩剂10 mL灌胃,对照组以生理盐水10 mL灌胃,然后制作兔大脑中动脉栓塞-再灌注模型。24 h后处死,制作大脑切片。TTC染色测量梗死面积,肉眼观察是否有再灌注出血。结果实验组发生出血5例,对照组发生出血19例。2组比较有显著性差异(P<0.05)。结论制半夏煎剂具有减少兔脑梗死再灌注出血转化的作用。

Objective It is to study the effect of Pinellia decotion on decreasing hemorrhagic transformation in rabbits with cerebral infarction and reperfusion.Methods 106 New Zealand rabbits were randomly divided into experimental group and control group.The experimental group was given concentration of Pinellia decoction 10 mL by intragastric administration at 2 h before experiment,and the control group was given normal saline at the same time by intragastric administration.Then middle cerebral artery occlusion-reperfusion models of rabbits were established.All the rabbits were killed after 24h to make brain slices.Infarct size after TTC staining were measured and whether reperfusion hemorrhage occurred was judged by visual observation.Results There were 5 hemorrhage cases in the experimental group,while there were 19 cases in the control group.Compared with each other,the difference was significant（P〈0.05）.Conclusion Pinellia decoction has a function that can decrease hemorrhagic transformation in rabbits with cerebral infarction and reperfusion.