摘要
目的初步考察煮制温度和时间对白芍中芍药苷含量的影响;测定煮制与未煮制、地下部分不同部位芍药苷的含量变化。方法采用高效液相色谱法,色谱条件:LichroCARTRP-C18(4mm×250mm,5μm);流动相:乙腈-0.1%磷酸溶液(14:86);流速:1mL.min-1;检测波长:230nm;柱温:30℃;进样量:10μL。结果煮制温度和时间对芍药苷含量变化的影响较大;不同品种的白芍85℃煮制15min后芍药苷的含量均高于煮制前芍药苷含量;白芍地下各部分芍药苷变化趋势为根茎(芍头)>须根>主根。结论白芍的煮制程度应有明确指标;应系统全面研究白芍和赤芍的化学成分和药效学,不应仅凭一个指标来考证;白芍是否去皮应明确化。
Objective To study the method of preparation and quality control of loxoprofen sodium plastic.Methods Loxoprofen sodium plastic was prepared with chitosan as plastic material and high performance liquid chromatography（HPLC） was used to determine the content of loxoprofen sodium.Results The linear range of loxoprofen sodium was 10-90 μg.mL-1,while the average recovery rate was 100.22%,and RSD was 0.44%（n=9）.Conclusion The preparation technology for loxoprofen sodium plastic is reasonable,and quality control is feasible,both of which are consistent with relevant regulations of plastic preparation of China pharmacopoeia（2005 edition）.
出处
《医药导报》
CAS
2011年第1期82-85,共4页
Herald of Medicine
基金
国家科技支撑计划基金资助项目(基金编号:2006BAI06A12-07)
关键词
白芍
芍药苷
煮制
Loxoprofen sodium
Plastic
Preparation
HPLC
Quality control
