肺结核56例痰标本FQ-PCR检测价值分析

Compared study of sputum specimen from patient with pulmonary tuberculosis detected with fluorogenic probes and realteme PCR

目的:探讨不同检测方法对肺结核的诊断价值。方法:以实时荧光定量聚合酶链反应法、痰直接涂片找抗酸杆菌法、痰结核杆菌培养法对56例确诊肺结核患者痰标本检查结果进行比较。结果:荧光定量PCR法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法(P<0.05),其他非肺结核结果阳性率仅为2.7%,特异度较高。结论:实时荧光定量聚合酶链反应法对肺结核诊断方面灵敏度及特异度较高,同时反映抗结核治疗过程中痰标本中的结核杆菌的数量变化,对抗结核药物疗效有良好的监控效果。

Objective： To discuss the diagnosis value of different methods on pulmonary tuberculosis.Methods ：The sputum specimens obtained from 56 patients with tuberculosis were detected mycobacterium tuberculosis（MTB） with fluorogenic probes and real-time PCR（FQ-PCR）,smear acid-fast and cultureof organism. Results ：Thepositive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and cultureof organism.Thepositive rate of non-tuberculosis was only 2.7 %.The specificity was higher.Conclusion： The high sensitivity and specificity rate renders a positive FQ-PCR result,which isclinically useful. Furthermore,the variationof the TB-DNA reflects the variationof the mycobacterium tuberculosis when treatment.It is useful to monitor the curative effect.