人参养荣汤对胰岛素刺激的大鼠脂肪细胞瘦素表达的影响

The Effects of Renshen Yangrong decoction on Leptin Expression in Insulin-induced Rat Adipocytes

目的:观察人参养荣汤对胰岛素刺激的大鼠脂肪细胞瘦素(Leptin)分泌及其mRNA表达的影响。方法:制备人参养荣汤含药血清及阳性对照开同含药血清,用含药血清处理经胰岛素刺激的脂肪细胞,放免法检测上清Leptin分泌水平,RT-PCR法检测细胞LeptinmRNA表达水平。结果:经胰岛素刺激后脂肪细胞Leptin表达显著增加,加入人参养荣汤含药血清后脂肪细胞Leptin的表达显著受到抑制。结论:人参养荣汤可抑制胰岛素刺激引起的Leptin的过度表达。

Objective： To observe the effect of Renshen Yangrong decoction on Leptin secretion and mRNA expression in insulin-induced rat adipocytes.Methods： Rat sera containing herbs or Kai Tong were collected after rats were intragastricly administered with the Renshen Yangrong decoction or Kai Tong respectively.Adipocytes induced by insulin were incubated with heat-inactivated pharmacologic serum.The Leptin secreted in culture supernatant were measured by radio immunoassay.The expression of LeptinmRNA in adipocytes was detected by RT-PCR.Results： Insulin increased the expression of Leptin in adipocytes significantly,Renshen Yangrong decoction inhibited the excessive expression of Leptin in adipocytes induced by insulin significantly.Conclusion： Renshen Yangrong decoction can inhibit the excessive expression of Leptin in adipocytes induced by insulin.