摘要
为研究产碱性磷酸酶(ALP)细菌对微囊藻(Microcystis aeruginosa)从有机磷中获得磷营养的影响,从微囊藻群体中分离获得一株产ALP附生菌Y6,通过ITS分子鉴定该菌属赤杆菌属(Erythrobacter).探讨该菌在有机磷条件下对微囊藻生长及生理代谢的影响,结果表明:(1)细菌发酵液中ALP活性与Y6菌的浓度呈正相关,在培养初期的延滞期即开始产生ALP;(2)在有机磷源条件下Y6的加入对微囊藻生长有促进作用;(3)有机磷源条件下,Y6有助于维持群体微囊藻的上浮率和较大群体,Y6的加入也有维持藻细胞叶绿素荧光强度的作用.本研究的结果表明,产ALP附生菌有助于微囊藻利用水体中有机磷,有利于水华形成.
To study the effect of alkaline phosphatase(ALP) bacteria on Microcystis aeruginosa obtaining phosphorus from organic phosphorus compounds,an attached bacterium strain Y6 which produced ALP was isolated from M.aeruginosa colonies.It was identified as a strain of Erythrobacter by its ITS molecular characteristic.The effects of the bacterium on the growth and physiology of M.aeruginosa in the organic phosphorus condition investigated in this paper.The results show that:(1) ALP activity in the culture broth was positively related Y6 concentration,initial lag phase of incubation began to produce ALP;(2) Under the condition of organic phosphorus,Y6 could promote the growth of M.aeruginosa;(3) Y6 could maintain floating rate and bigger colonies of colonial M.aeruginosa,and hold chlorophyll fluorescence of algal cells in the organic phosphorus medium.This research suggested that ALP producing attached bacteria could help M.aeruginosa using organic phosphorus and forming blooms.
出处
《湖泊科学》
EI
CAS
CSCD
北大核心
2011年第1期49-55,共7页
Journal of Lake Sciences
基金
江苏省科技厅社会发展项目(BS200765)
国家重点基础研究发展计划“973”项目(2008CB418004)联合资助
关键词
微囊藻
藻上附生菌
碱性磷酸酶
Chl.a荧光强度
水华
Microcystis aeruginosa
attached bacteria
alkaline phosphatase
Chl.a fluorescence intensity
water bloom