自血穴位注射对哮喘小鼠肺组织Bcl-2及Fas表达的影响

Effect of Autologous Blood Acupoint Injection on Bcl-2 and Fas Expression in Asthmatic Mouse Lung Tissue

【目的】观察自血穴位注射对哮喘小鼠肺组织凋亡抗原Fas与抗凋亡抗原Bcl-2表达的影响。【方法】选用BALB/c小鼠,随机分为正常对照组、模型组、生理盐水穴位注射组、自血穴位注射组、地塞米松组;除正常对照组外,其他组均采用卵蛋白注射致敏加超声雾化吸入法复制哮喘模型;自血穴位注射组采用自体血注射两侧定喘穴,每穴0.1 mL,生理盐水穴位注射组则以生理盐水代替注射,地塞米松组以0.5 mg/kg剂量腹腔注射,均隔天1次,1周为1个疗程。采用免疫组化法观察各组小鼠肺组织Fas与Bcl-2表达,并观察各组小鼠肺组织病理形态变化。【结果】模型组Fas与Bcl-2的面密度均显著升高(P<0.05或P<0.01);自血穴位注射组与地塞米松组均可显著升高Fas面密度,降低Bcl-2面密度,与模型组比较差异均有显著性意义(P<0.05),而2组间比较差异无显著性意义(P>0.05);生理盐水穴位注射组与模型组比较差异无显著性意义(P>0.05)。自血穴位注射组可一定程度改善哮喘小鼠的肺组织病理形态变化。【结果】自血穴位注射疗法治疗哮喘的作用可能与上调哮喘小鼠肺组织Fas表达,下调Bcl-2表达,促进炎症细胞凋亡,改善气道炎症有关。

Objective To observe the effect of autologous blood acupoint injection on apoptosis antigen Bcl-2 and anti-apoptosis antigen Fas expression in asthmatic mouse lung tissue. Methods BALB/c mice were randomized into normal control group, model group, normal saline acupoint injection group, autologous blood aeupoint injection group and dexamethasone group. Except for the normal control group, the mice in other groups were sensitized with intraperitoneal injection of albumin and challenged by untrasonic inhalation of albumin. On bilateral Dingchuan （EX-B1） points, autologous blood acupoint injection group was given injection of autologous blood 0. 1 mL, normal saline acupoint injection group was given normal saline, and dexamethasone group was given intraperitoneal injection of dexamethasone 0.5mg/kg, once every other day. The treatment lasted one week. Bcl-2 and Fas expression in mouse lung tissue was examined by immnuohistochemical assay. The pathological changes of mouse lung tissue were also observed. Results Compared with the normal control group, the area density of Bel-2 and Fas in mouse lung tissue of model group was increased （P 〈0. 05 or P 〈0.01 ）. The area density of Fas was higher and that of Bcl-2 was lower in autologous blood acupoint injection group and dexamethasone group than those in the model group （P 〈0.05 ）. The differences of the area density of Bel-2 and Fas were insignificant between autologous blood aeupoint injection group and dexamethasone group, and insignificant between normal saline acupoint injection group and model group （P 〉 0. 05 ）. In autologous blood acupoint injection group, the pathological changes of mouse lung tissue were relieved to some extent. Conclusion The mechanism of autologous blood acupoint injection is probably related with the up-regulation of Fax expression and the down-regulation of Bcl-2 expression, and with the promotion of inflammatory cells apoptosis and the improvement of air way inflammation.