摘要
研究了红酵母(Rhodotorula sp.8)利用霉菌的玉米粉水解液合成类胡萝卜素的过程。结果表明,黑曲霉AQ72的玉米粉发酵液适于红酵母R.sp 的生长及类胡萝卜素的合成。在60 g/L玉米粉为底物,经黑曲霉水解后,摇瓶震荡培养红酵母84 h,生物量干细胞达13.4 g/L,色素含量为3.42 m g/L。经高效液相色谱测定,其色素中,含量最高的是β-胡萝卜素,达2.67 m g/L。
Biosynthesis of carotenoid by the two steps fermentation of the red yeast Rhodotorula sp. with various moulds in corn powder broth was studied. The results show that Aspergillus niger Q72 is suitable to the first period fermentation. Using corn powder (60g/L) as substance, the red yeast was cultured on shaker for 84 hours. The biomass of dried cell (13.4g/L) and the total yield of carotenoid (3.42mg/L) were obtained. By determination of high pressure liquid chromatography (HPLC), the main carotenoid pigment was β carotene as much as 2.67mg/L . The research provides a new way of pigments production by biosynthesis stead of chemosynthesis.
出处
《清华大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
1999年第10期101-103,共3页
Journal of Tsinghua University(Science and Technology)