红酵母sp.8生物合成类胡萝卜素的研究

Study on biosynthesis of carotenoid using Rhodotorula sp.8

研究了红酵母（Ｒｈｏｄｏｔｏｒｕｌａ ｓｐ．８）利用霉菌的玉米粉水解液合成类胡萝卜素的过程。结果表明，黑曲霉ＡＱ７２的玉米粉发酵液适于红酵母Ｒ．ｓｐ 的生长及类胡萝卜素的合成。在６０ ｇ／Ｌ玉米粉为底物，经黑曲霉水解后，摇瓶震荡培养红酵母８４ ｈ，生物量干细胞达１３．４ ｇ／Ｌ，色素含量为３．４２ ｍ ｇ／Ｌ。经高效液相色谱测定，其色素中，含量最高的是β－胡萝卜素，达２．６７ ｍ ｇ／Ｌ。

Biosynthesis of carotenoid by the two steps fermentation of the red yeast Rhodotorula sp. with various moulds in corn powder broth was studied. The results show that Aspergillus niger Q72 is suitable to the first period fermentation. Using corn powder (60g/L) as substance, the red yeast was cultured on shaker for 84 hours. The biomass of dried cell (13.4g/L) and the total yield of carotenoid (3.42mg/L) were obtained. By determination of high pressure liquid chromatography (HPLC), the main carotenoid pigment was β carotene as much as 2.67mg/L . The research provides a new way of pigments production by biosynthesis stead of chemosynthesis.