甘蓝型油菜萝卜质不育系恢复材料Ad-6(F_4)测交二代恢复株TCF_2的快繁及生根

THE REGENERATION OF POLYGEMMIC AND ROOTS OF THE SECOND GENERATION RESTORER TCF_2 WHICH WERE TEST CROSSINGBETWEEN BRASSICA NAPUS OguCMS AND THE RESTORER Ad 6(F_4)

油菜萝卜胞质不育系恢复材料Ａｄ－６（Ｆ４）测交二代恢复株ＴＣＦ２，在ＭＳ附加５ｍ ｇ／Ｌ６－ＢＡ＋ ０．５ｍ ｇ／Ｌ ＮＡＡ，３ｍ ｇ／Ｌ ６－ＢＡ＋ ０．２ｍ ｇ／Ｌ ＮＡＡ，３ｍ ｇ／Ｌ ６－ＢＡ＋ ０．３ｍ ｇ／Ｌ ＮＡＡ＋６００ｍ ｇ／Ｌ水解乳蛋白以及５ｍ ｇ／Ｌ６－ＢＡ＋ ０．３ｍ ｇ／ＬＮＡＡ＋ ５ｍ ｇ／ＬＡｇＮＯ３ 的不同培养基上诱导丛生芽，结果表明５ｍ ｇ／Ｌ６－ＢＡ＋ ０．３ｍ ｇ／ＬＮＡＡ＋ ５ｍ ｇ／ＬＡｇＮＯ３ 对ＴＣＦ２ 诱导丛生芽有较好的效果．小芽生根培养基为１／２ＭＳ效果最好．

The restorer TCF 2s which came from the second gereration of test crossing between Brassica napus OguCMS and restorer Ad 6(F 4) were cultured in MS medium added 5mg/L 6 BA+ 0.5 mg/L NAA,3mg/L 6 BA+0.2mg/L NAA,3mg/L 6 BA+0.3mg/L NAA+600mg/L LH and 3mg/L 6 BA+0.3mg/L NAA+5mg/L AgNO 3 to induce the polygemmic.The results showed that medium added 5mg/L 6 BA+0.3mg/L NAA+5mg/L AgNO 3 had the good result to induce the polygemmic.And 1/2MS medium had the good result to induce the roots of the shoots.