摘要
栽培大豆和野生大豆叶片的蛋白抽提液经硫酸铵盐析、Sephadex G-50柱层析以及浓缩透析等步骤,分别得到核酮糖-1,5-二磷酸羟化酶(简称Rubisco)的结晶.经SDS-PAGE和Western blot酶标抗体技术鉴定证实为Rubisco.用IEF-PAGE方法分析这2种不同进化类型大豆的Rubisco 大小亚基。
Rubisco (Ribulose 1,5 bisphosphate Caboxylase) was purified and crystallized from Glycine max and Glycine soja leaves. The results of SDS PAGE and Western blot confirmed that the crystalline proteins were Rubisco. Electrofocusing analysis of large and small subunits of Rubisco from G. max and G. soja indicated that both proteins had identical isoelectric focusing patterns consisting of three large and two small subunit polyeptides.
出处
《复旦学报(自然科学版)》
CAS
CSCD
北大核心
1999年第5期533-536,共4页
Journal of Fudan University:Natural Science
基金
国家自然科学基金