栽培和野生大豆核酮糖-1,5-二磷酸羧化酶的结晶及其大小亚基等电聚焦分析

Crystallization of Rubisco and Its Isoelectrofocusing Analysis of Large and Small Subunits from Wild and Cultivated Soybean

栽培大豆和野生大豆叶片的蛋白抽提液经硫酸铵盐析、Ｓｅｐｈａｄｅｘ Ｇ－５０柱层析以及浓缩透析等步骤，分别得到核酮糖－１，５－二磷酸羟化酶（简称Ｒｕｂｉｓｃｏ）的结晶．经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ ｂｌｏｔ酶标抗体技术鉴定证实为Ｒｕｂｉｓｃｏ．用ＩＥＦ－ＰＡＧＥ方法分析这２种不同进化类型大豆的Ｒｕｂｉｓｃｏ 大小亚基。

Rubisco (Ribulose 1,5 bisphosphate Caboxylase) was purified and crystallized from Glycine max and Glycine soja leaves. The results of SDS PAGE and Western blot confirmed that the crystalline proteins were Rubisco. Electrofocusing analysis of large and small subunits of Rubisco from G. max and G. soja indicated that both proteins had identical isoelectric focusing patterns consisting of three large and two small subunit polyeptides.