摘要
在农杆菌菌液与植物材料混合培养时辅以Ｔｗ ｅｅｎ ２０和负压处理，用ｇｕｓ报告基因的瞬间表达作为指标，与仅用Ｔｗ ｅｅｎ２０或仅用负压处理的样本进行了比较研究。实验结果表明：适当的负压处理可提高转化频率，而过度的负压处理会对材料造成较大的伤害，从而降低其成活率，如同时使用Ｔｗ ｅｅｎ２０可以减轻这种伤害。
When the cell suspension of Agrobacterium tumefaciens was mixed and co cultured with plant cells, the mixture was disposed using Tween 20 and negative pressure simultanecously. According to the transient expression of gus report gene in the callus treated, the effect of the disposition was compared with that of using Tween 20 or negative pressure only. The results showed that appropriate treatment could increase the transformation frequency and survival rate while over treatment harmed plant cells and decreased the survival rate. Addition of Tween 20 while using negative pressure could decrease the harmness caused by the over treatment.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1999年第5期601-604,共4页
Journal of Fudan University：Natural Science
基金
国家科委八六三高科技项目
洛氏基金
关键词
农杆菌
转化
水稻
Tween20
Agrobacterium tumefaciens
transformation
rice
negative pressure
Tween 20
