HPLC法测定洋蓟茎叶提取物中绿原酸与洋蓟素的含量

Chlorogenic Acid and Cynarin in Extracts of the Cynara Scolymus L. Leaf and Stem by HPLC

建立了测定洋蓟提取物中绿原酸与洋蓟素含量的高效液相色谱法。采用高效液相色谱法,色谱柱C-18(150mm×4.6mm,5μm);流动相A为乙腈,B为0.2%磷酸水,采用梯度洗脱30min,乙腈与磷酸水体积比从5∶95至50∶50;检测波长为330nm;流速:1mL/min;柱温:30℃;进样量:10μL。结果表明,绿原酸为0.13～0.66μg,线性关系良好,洋蓟素为0.12～0.62μg,线性关系良好,相关线性系数均为R=0.9999,绿原酸平均回收率为99.6%,RSD为0.41%;洋蓟素平均回收率为99.4%,RSD为0.31%;绿原酸与洋蓟素峰的理论塔板数均大于10000。该方法操作简单、准确、重复性好,适用于洋蓟茎叶提取物中绿原酸与洋蓟素含量的测定。

HPLC method for the analysis of the chlorogenic acid and cynarin is developed in extracts of the Cynara scolymus L. leaf and stem. C18 column （waters summetry, 150mm×4.6mm,5μm） is used with a gradient elution, with the mobile phase from acetonitrile： 0.2% phosphoric acid in water 5 ： 95 to 50 ： 50 in 30 min, and the wavelength of detecter is set at 330 nm. The results show that the linear range of the chlorogenic acid and cynarin is 0.13-0.66 μg and 0.12-0.62μg respectively. The average recovery of the chlorogenic acid and cynarin is 99.6% and 99.4% respectively, RSD is 0.41% and 0.31% （n=9） respectively. This method is rapid, accurate and reproducible for analysis of chlorogenic acid and cynarin in extracts of the Cynara scolymus L. leaf and stem.