以壳聚糖微球为载体对β-葡萄糖苷酶固定化的研究

Immobilization of β-Glucosidase on Chitosan Beads

以壳聚糖微球为载体,采用包埋—交联法固定化β-葡萄糖苷酶。通过单因素和正交试验确定了最佳固定化方法。研究结果表明,β-葡萄糖苷酶的最佳固定化条件为:pH值4.6,温度65℃,酶用量166.88IU/g(以绝干壳聚糖计,下同)的β-葡萄糖苷酶经质量分数为2.5%的壳聚糖微球包埋2h,在25℃下质量分数为4.0%的戊二醛下交联2h,得到酶活力为152IU/g和酶活回收率为90.60%的固定化酶。

β- glncosidase is immobilized on chitosan beads by embedding and crosslinking. The best immobilization method is determined by the single and the orthogonal test. The optimal conditions of immobilization is as follows： β-glucosidase dosage 166.88 IU/g dry chitosan is embedded with concentration of 2.5% ehitosan beads at pH value 4.6 and temperature 65 ℃ for 2 h, followed by crosslinking at 25 ℃ for 2 h in 4.0% glutaraldehyde （GA） solution. The activity of immobilized β-glucosidase and recovery rate of β-glucosidase activity are 152 IU/g （based on dry chitosan） and 90.60%, respectively.