当归贝母苦参丸对良性前列腺增生模型小鼠PCNA、bcl-2和bax表达的影响

Effects of Danggui-beimu-kushen Pill on expression of PCNA, bcl-2 and bax in BPH mice model

目的探讨当归贝母苦参丸对良性前列腺增生（BPH）模型小鼠的影响及作用机理。方法将60只雄性小鼠，按照随机数字表法随机分为6组：空白组，模型组，阳性对照组，高、中、低剂量治疗组。卒白组给予腹腔注射12．5mg／kg·d-1橄榄油，其他各组采用腹腔注射丙酸睾酮原液制作小鼠BPH模型，同时高、中、低剂量治疗组分别予高、中、低剂量当归贝母苦参丸水煎溶液灌胃，阳性对照组灌胃前列康水混：悬液600mg／kg。给药第21大处死，观察前列腺指数（PI）的变化；HE染色光镜观察前列腺病理改变；免疫组化方法检测PCNA、bcl-2和bax表达的变化。结果当归贝母苦参丸治疗组PI低于模型组；PCNA、bcl-2表达IOD值低于模型组：bax表达高于模型组。治疗组高、中、低剂量组比较差异无统计学意义，三个治疗组与阳性对照组比较筹异无统计学意义。结论当归贝母苦参丸可抑制模型小鼠BPH，其机理可能与下调细胞增殖蛋白PCNA和凋亡抑制蛋白bcl-2、增加bax的表达相关。

Objective To study the effect of Danggui-beimu-kushen Pill on benign prostatic hyperplasia （BPH） and its possible mechanism. Methods BPH model mouse was produced by intraperitoneal injection of testosterone propionate. The high, medium and low dose treatment group was fed high, middle and low dose of Danggui-beimu-kushen Pill respectively, while the positive control group was fed Qianliekang liquid, 600 mg/kg. All mice were executed on the 21 day. Such values were observed as the prostate index changes, pathological changes of prostate by HE staining light microscopy and the expression changes of PCNA, bcl-2 and bax by immunohistochemistry. Results The prostate index of the treatment group was lower than the model group; the PCNA and bcl-2 expression were lower than the model group, while the bax expression was higher than the model group. Meanwhile; there is no significant difference among the treatment groups. Moreover, the difference between the treatment groups and the positive control group has no statistic meaning. Conclusion Danggui-beimu-kushen Pill can suppress BPH in mice. The mechanism may be related to the reduction expression of cell-proliferation protein PCNA and apoptosis profilin bcl-2, and the increase of bax expression.