不同处理对西藏牦牛超数排卵的影响研究

An Investigation of Efficacy of Different Reproductive Techniques to Induce Multiple-ovulation in Tibetan Yaks

本研究选用了埋植CIDR栓法、埋植PRID栓法、PG法以及PMSG+PG法四套方案对西藏41头母牦牛进行了超数排卵试验。试验结果:埋植CIDR法得到的平均卵母细胞数为7.4枚/头,埋植PRID法得到的平均卵母细胞数为6.5枚/头,PG法得到的平均卵母细胞数为4枚/头,PMSG+PG法得到的平均卵母细胞数为4.8枚/头,各组之间差异极显著(P<0.01);而经过卵母细胞成熟培养之后,埋植CIDR法得到的平均可用卵母细胞数为6.2枚/头,埋植PRID法得到的平均可用卵母细胞数也为6.2枚/头,PG法得到的平均可用卵母细胞数为4枚/头,PMSG+PG法得到的平均可用卵母细胞数为4.6枚/头,CIDR+FSH法与PRID+FSH法两组、两次PG法与PMSG+PG法两组差异不显著(P>0.05),但是CIDR+FSH法和PRID+FSH法组与两次PG法和PMSG+PG法组之间差异都极显著(P<0.01);用CIDR法和PRID法进行西藏牦牛超排效果较好,并可获得较多的可用卵母细胞。

The following paper investigates the efficacy of four different reproductive techniques to induce multiple-ovulation in 41 Tibetan yaks. The different treatments used included Controlled Internal Drug Releasing Device （CIDR）, Progesterone Releasing Intravaginal Device （PRID）, Prostagtandin （PG） injection, and a combination of Pregnant Mare Serum Gonadotrophin and Prostaglandin （PMSG ＋ PG） injection. The average number of oocytes varied significantly between the four treatments （ P 〈 0.01） ： 7.4 with CIDR, 6.5 with PRID, 4 with PG and 4.8 with PMSG ＋ PG, After oocyte maturation, the average number of available oocytes according to treatment was 6.2 for both CIDR and PRID methods, 4 for PG, and 4.6 for PMSG ＋PG. There were no significant differences between the CIDR and PRID methods （P 〉 0.05）. Similarly, there were no significant differences between the PG and PMSG ＋ PG methods （P 〉 0.05）. Furthermore, the number of viable oocytes was significantly higher when using CIDR and PRID compared to PG and PMSG ＋PG （P 〈 0.01）. The tollowing study recommends the use of internal hormone-based devises （CIDR or PRID） for inducing multiple-ovulation in yaks.