反向斑点杂交-基因芯片检测人类乳头瘤病毒及分型

Clinical application of detecting and typing human papillomavirus with dot blot hybridization reverse-gene chip

目的检测人类乳头瘤病毒（humanpa pilloma virus，HPV）及其分型，了解其在女性宫颈中的感染情况与宫颈癌防治方面的意义。方法采用反向斑点杂交．基因芯片检测220例不同年龄患者宫颈中HPV感染情况与分型情况，对比分析低危型与高危型HPV亚型感染率以及其与年龄的关系。结果220例标本中HPV感染阳性率为12．3％，其中单一型感染阳性率为10．9％，两种亚型同时感染率为1．4％；高危亚型与低危亚型的阳性率分别为10．0％与2．3％，二者比较有统计学意义（P〈0．05）；20-30岁年龄组、30-40岁年龄组、40～50岁年龄组与50岁及以上年龄组HPV感染率分别为13．0％、12．3％、4．9％与35．3％（P〈0．05），HPV高危亚型感染率分别为8．7％、9．6％、4．9％与35．3％，差异具有统计学意义（P〈0．05）。结论反向斑点杂交一基因芯片可以准确、快速地对HPV进行分型，为宫颈癌的防治与HPV流行病学研究奠定了技术基础。

Objective To detect and type human papillomavirus（HPV） by the method of dot blot hybridization reverse-gene chip for investigating their infections in women and their roles in the prevention and treatment in cervical cancer. Methods 220 patients from different age were enrolled and the human papillomavirus（HPV） were detected and typed by dot blot hybridization reverse-gene chip. The infection rate of low-risk and high-risk types were compared and the relationship with age were analyzed. Results The positive rate of HPV from 220 patients was 12.3%, the single positive infection was 10.9%, while the infection of two kinds of HPV subtypes was only 1.4%, the positive rate of high-risk types and low-risk types were 10.0% and 2.3% respectively（P〈0.05）. The positive rate of HPV were 13.0%, 12.3%, 4.9% and 35.3% along with the age group of 20-30, 30-40, 40-50 and ≥ 50 years respectively（P〈0.05）, the positive rate of high-risk types HPV were 35.5% in 〉 50 years, which was singnificanly higher than the age of 20-30（8.7%）, 30-40（9.6%） and 40-50（4.9%） （P〈0.05）. Moreover, the patients with positive HPV were all high-risk types HPV in the age group 40-50 and 〉 50 years. Conclusion The HPV gene subtypes can be detected and typed accurately and quickly by dot blot hybridization reverse-gene chip. It provided the foundation for prevention of cervical cancer and epidemiological studies of HPV.