胸腺内注射异基因抗原诱导同种异体肢体移植免疫耐受的实验研究

The study of immune torlerance induced by intrathymic injection of allogenic antigen in rat limb allotransplantation model

目的:探讨胸腺内注射异基因抗原对同种异体肢体移植存活的影响。方法:Wistar大鼠为供体,SD大鼠为受体。将SD大鼠随机分成4组,A组(自体肢体移植组)、B组(异体肢体移植组)、C组(异体肢体移植加用免疫抑制剂组)、D组(胸腺内注射组)。术后观察肢体的存活时间,术后7d进行组织学检查。结果:肢体存活时间C组和D组比较,差异无统计学意义(P>0.05),与B组比较,差异有统计学意义(P<0.01)。组织学检查,D组优于B、C组(P<0.05)。结论:胸腺内注射异基因抗原可诱导对异体肢体移植的特异性免疫耐受。

Objective： To study the immune torlerance inductive effects by intrathymic injection of allogenic antigen in rat limb allotransplantation model. Methods： Wistar rats were used as donators and SD rats as recipients.SD rats were divided into four groups, group A （autogenic limb graft group）, group B （allogeneic limb graft group）, group C （allogeneic limb graft plus immune degressant group）, group D （allogeneic limb graft plus intrathymical injection group）. The survival time of limb grafts was observed after operation. The histological study was also followed after 7 days post operation. Results： The survival time of limb grafts showed that group D and group C were not statistically significant （P〉0.05）, however, group D and group B were statistically significant （P〈0.01）. Histological study showed that group D surpassed the effects of group B and group C. Conclusion： Intrathymic injection of allogenic antigen could induce immune torlerance effectively in rat limb allotransplantation model.