食管鳞癌患者癌及癌旁组织差异基因表达

Difference in gene expression profiles of both squamous esophageal cancer tissues and adjacent normal tissues

目的:探讨食管鳞癌组织差异基因表达。方法:分别取3例无食管鳞癌家族史患者的癌及癌旁组织(Ⅰ组)和6例有食管鳞癌家族史患者的癌及癌旁组织(Ⅱ组)等量混合,抽提RNA,将RNA逆转录合成相应cDNA,以Cy5和Cy3标记cDNA作为探针,在含有14 000点人类基因组芯片(BiostarH-140s)上进行杂交。用scanarray 4000扫描仪扫描芯片荧光信号图像,用GenePix Pro 3.0软件对扫描图像进行数字化处理和分析。结果:依Ratio(Cy5/Cy3)>2.0或<0.5的数据项为差异表达的基因,Ⅰ组和Ⅱ组分别为1 855个和613个。两组具相同异常表达的基因有60个,含表达序列标签(EST)30个。30个基因中,除上皮细胞膜蛋白-1、骨桥素、人类泛素耦联酶E2C、成视网膜细胞瘤样-2共4个基因外,其余26个基因中未见与食管癌相关的报道。结论:利用基因芯片法可高通量地筛选出散发和有食管鳞癌家族史患者癌组织中共同存在的异常表达的基因谱,为进一步阐明食管癌的发病机制提供信息。

Objective：To investigate the gene expression profiles of squamous esophageal cancer carcinoma（ESCC）.Methods：The cDNA retro-transcribed from both 3 cases（groupⅠ） and 6 cases（groupⅡ）,which consisted of equal quantity of both squamous esophageal cancer tissues and adjacent normal tissues,were labelled with Cy5 and Cy3 fluorescence as probes.The mixed probes were hybridized with a piece of Biostar H-140s single dot human whole gene chip.It was scanned by scanarray 4 000 instrument.The acquired image was analyzed by GenePix Pro 3.0 software.Results：There were 1 855 genes in groupⅠ and 613 genes in the group Ⅱ,respectively,whose Cy5/Cy3 ratio was greater than 2.0 or less than 0.5.Furthermore,there were 60 genes,whose Cy5/Cy3 ratio wasgreater than 2.5 or less than 0.25 simultaneously in bothⅠgroup and Ⅱ group,including 30 ESTs in GenBankTM.There are 26 genes that has not been reported relating to squamous esophageal cancer,except 4 genes of epithelial membrane protein 1（EMP1）,secreted phosphoprotein 1（SPP1）,ubiquitin-conjugating enzyme E2C（UBE2C） and retinoblastoma-like 2（p130）（RBL2）.Conclu-sion：Microarray may play important roles in exploring the gene change in ESCC with and without family history,to illustrate the mechanism on the developement of ESCC.