子痫前期内质网应激相关蛋白CHOP/GADD153的表达及其意义

Expression and significance of CHOP / GADD153 related ERS in preeclampsia

目的:研究内质网应激相关蛋白CHOP/GADD153在子痫前期患者胎盘组织中的表达及其意义。方法:选择2009年6月～2010年6月在南京医科大学第一附属医院产科住院分娩的子痫前期患者30例(子痫前期组),以同期正常孕妇35例为对照组。采用RT-PCR技术、蛋白印迹(western-blot)法和免疫组化检测两组孕妇胎盘组织中CHOP/GADD153的mRNA与蛋白表达水平差异。结果:子痫前期组患者胎盘组织中CHOP/GADD153的mRNA与蛋白表达水平均显著高于相应孕周正常妊娠组。结论:CHOP/GADD153表达的增高与子痫前期的发病相关。

Objective：To investigate the expression and significance of C / EBP homologous protein / growth-arrest and DNA damage inducible gene 153（CHOP / GADD153） in preeclampsia.Methods： Semiquantitative RT-PCR,immunohistochemistry and Western-blot were used to investigate the expression of CHOP / GADD153 mRNA and protein levels in placenta of 30 women with preeclampsia and 35 normal pregnant women.Results： ①The expression of CHOP / GADD153 mRNA was significantly higher in preeclampsia group（13.416 ± 1.516） than in the normal pregnant group（2.615 ± 0.659,P 〈 0.01）.②The mean density of CHOP / GADD153 in preeclampsia placenta（0.156 ± 0.008） was significantly higher than that in the th normal pregnant women group（0.143 ± 0.009,P 〈 0.01）.③The expression of CHOP / GADD153 protein levels was significantly higher in preeclampsia group（16.210 ± 1.217）than in the normal pregnant group（2.710 ± 0.799,P 〈 0.01）.Conclusion：Up regulation CHOP / GADD153 mRNA and excess expression of CHOP / GADD153 protein levels in placenta may be involved in the pathophysiological processes of preeclampsia.