摘要
目的建立同时测定镇江巴布膏中冰片、薄荷脑、樟脑和水杨酸甲酯含量的方法。方法采用GC法,以萘为内标物,色谱柱为HP-INNOMAX毛细管柱(30 m×0.32 mm,1.0μm),进样口温度为230℃,FID检测器温度为250℃;采用程序升温,起始温度为90℃,保持7 min,以5℃·min-1的速率升至140℃,保持13 min。结果龙脑、异龙脑、樟脑、薄荷脑和水杨酸甲酯的线性范围分别为0.033 0~0.329 8 mg/mL(r=0.999 0)、0.015 9~0.158 8 mg/mL(r=0.999 0)、0.050 7~0.507 1 mg/mL(r=0.999 1)、0.060 0~0.599 8 mg/mL(r=0.999 2)、0.2470~2.470 4 mg/mL(r=0.999 0),平均加样回收率分别为99.12%、99.96%、100.53%、100.94%和104.58%,RSD均小于5%(n=6)。结论所建方法快速、简便、准确,可有效地控制镇江巴布膏的质量。
Objective To establish GC method for simultaneous determination of borneol,menthol,camphor and methylsalicylate contents in Zhenjiang Babu plaster.Methods The internal standard was Naphthalene.HP-INNOMAX quartz capillary column(30 m×0.32 mm,1.0μm) and FID were used.The injector temperature was 230℃ and the detector temperature was 250 ℃.Column temperature raised by programming with beginning temperature at 90℃,maintaining for 7 min,then increased the temperature to 140℃ at the rate of 5℃·min-1,maintaining for 13 min.Results The linear ranges of borneol,isoborneol,menthol,camphor and methylsalicylate were: 0.033 0~0.329 8 mg/mL(r=0.999 0),0.015 9~0.158 8 mg/mL(r=0.999 0),0.050 7~0.507 1 mg/mL(r=0.999 1),0.060 0~0.599 8 mg/mL(r=0.999 2),0.247 0~2.470 4 mg/mL(r=0.999 0),The average recoveries were 99.12%、99.96%、100.53%、100.94% and 104.58%,and all RSD were less than 5%(n=6).Conclusion This method is sensitive,accurate and reproducible.It can be used to control the quality of Zhenjiang Babu plaster.
出处
《现代中药研究与实践》
CAS
2011年第1期54-56,共3页
Research and Practice on Chinese Medicines
基金
江苏省科技成果转化专项资金项目(项目编号:BA2008099)