肝细胞癌组织HSP70、p53和PCNA的表达及其意义

Expression of heat shock protein 70,p53 protein and proliferating cell nuclear antigen in hepatocellular carcinoma

目的探讨热休克蛋白70(HSP70)、p53和增殖细胞核抗原(PCNA)在肝细胞癌(HCC)组织中的表达及其意义。方法采用免疫组化法检测正常人、慢性乙型肝炎、肝硬化和HCC肝或癌组织HSP70、p53和PCNA的表达情况。结果 HCC组织HSP70、p53和PCNA表达阳性率明显高于非癌组织(x21=27.16x,22=67.6,x23=40.6,P<0.01);HSP70在正常人、慢性乙型肝炎、肝硬化和HCC中的表达逐步增强;HSP70和p53在分化较好的HCC中的阳性率明显低于分化不良者(x21=6.8,P1<0.01x,22=6.1,P2<0.05),而PCNA表达与HCC组织分化程度无关(x2=2.4,P>0.05);HSP70表达强度与p53和PCNA表达关系密切(x21=41.3,x22=41.4,P<0.01)。结论 HCC是HSP70高表达肿瘤。HSP70表达与p53和PCNA表达密切相关,因而在HCC发生和发展中起重要作用。

Objective To invetstigate the expression and significance of heat shock protein 70（HSP70）,p53 and proliferating cell nuclear antigen（PCNA）in hepatocellular carcinoma（HCC）.Methods HSP70,p53 and PCNA were determined in liver tissues of control,chronic hepatitis B,liver cirrhosis and HCC by immunohistochemistry.Resultes The positive rates of HSP70,p53 and PCNA in HCC were higher than that in non-tumor tissues（x21=27.16,x22=67.6,x23=40.6,P〈0.01）;HSP70 expression increased progressively in liver tissues of control,chronic hepatitis B,liver cirrhosis and HCC;The positive rates of HSP70 and p53 in well-differenciated HCC were lower than that in poorly-differenciated HCC（x1^2=6.8,P1〈0.01,x2^2=6.1,P2〈0.05）;the expression of PCNA were not associated with histological grades（x^2=2.4,P〈0.05）;The expression of HSP70 was closely correlated to that of p53 and PCNA in HCC（x1^2=41.3,x2^2=41.4,P〈0.01）.Conclusion Expression of HSP70 is high in HCC.The expressions of HSP70,p53 and PCNA are closely correlated,which may play an important role in hepatocarcinogenesis.