牛血液中及蜱吸血后血液中牛瑟氏泰勒虫基因组DNA含量及同源性比较

Comparison about DNA Contents and Homology of Theileria. Sergenti in Ox Blood and after Tick Hemophagia Blood

利用姬姆萨染色法、PCR鉴定、紫外分光光度计法分别测定牛血液中及蜱吸血后血液中牛瑟氏泰勒虫DNA含量,对其进行比较分析。结果表明,蜱中DNA含量明显高于牛血液。根据牛瑟氏泰勒虫P33表面蛋白基因序列设计合成l对引物,以2种牛瑟氏泰勒虫基因组DNA为模板扩增,经序列测定,同源性比较分析,牛血液中和蜱血液中牛瑟氏泰勒虫基因序列同源性为100%,两者与GenBank上已发表的牛瑟氏泰勒虫序列同源性为99%,由此可见,该基因在蜱中和牛血液中具有相同的序列,进一步证明蜱是牛瑟氏泰勒虫病的传播媒介。为进一步研究由蜱为传播媒介的焦虫病、立克次氏体病等提供有力的材料基础,为该病的研究开拓了新的途径。

Determined DNA contents and homology of Theileria.sergenti in ox blood and after tick hemophagia blood using Giemsa s staining,PCR verification,UV spectrophotometer.The result showed that DNA contents of Theileria.sergenti higher obviously in tick s blood.Designed a couple of primer and amplified by follow board of two kinds of Theileria.sergenti DNA,according to GenBank publication P33 surface protein gene order after sequencing and analysis onhomology,their sequence homology were 100%,wihch were 99% with GenBank publication.It was clear that there was same sequences of Theileria.sergenti in ox blood and after tick hemophagia blood.Further proof the tick is important intermediary about Theileria.sergenti.Providing important fundament to research other disease such rickettsia and babesiosis.