慢性应激对小鼠空间学习记忆功能及脑内GDNF的影响

Effects of Chronic Stress on Ability of Spatial Learning-memory and Expression of GDNF in Mice′s Brain

探讨慢性应激对小鼠空间学习记忆功能的影响及其机制.通过Morris水迷宫试验测试小鼠的空间学习记忆能力;检测胶质细胞源性神经营养因子(GDNF)在海马和前脑皮层的表达.结果显示,应激组小鼠慢性应激后,与对照组相比,空间学习与记忆能力明显降低(P<0.01),海马CA1、CA3区、齿状回和前脑皮层GDNF表达明显下降(P<0.01);应激停止后一周,应激组小鼠空间学习与记忆能力有所恢复但仍弱于对照组小鼠,且有显著性差异(P<0.05);GDNF在各脑区的表达有所恢复,但与对照组相比,仍有显著性差异(P<0.05,P<0.01).结果提示,慢性应激导致小鼠空间学习记忆功能的损伤可能与GDNF表达的变化密切相关.

In this paper,we study the effects of chronic stress on the spatial learning-memory function of mice,and the expression of glial cell line-derived neurotrophic factor（GDNF） in mice＇s brain.The ability of spatial learning-memory of mice′s were determined by Morris water maze task,and the expression of GDNF in the hippocampus（HP） and prefrontal cortex（PFC） were detected by immunohistochemical method.The results showed that the ability of spatial learning and memory were significantly decreased（P0.01） in the stress group mice,compared with the control group mice.The GDNF expression in the CA1,CA3 and dentate gyrus of HP and PFC were significantly reduced in the stress group mice（P0.05,P0.01） on after stress and 7 days after stress period.The results suggested that chronic stress causes the damage of spatial learning and memory function in mice which may be nearly related to the changes of GDNF expression.