摘要
以西伯利亚花楸幼嫩茎段为外植体,0.1%(m/v)升汞消毒6 m in,在MS+6BA 0.8 mg/L+NAA 0.08 mg/L+KT 0.4 mg/L+IBA 0.04 mg/L的增殖培养基上,成功地建立了西伯利亚花楸试管苗增殖体系。研究了不同保存条件和培养基对试管苗保存效果的影响,结果表明:试管苗在(2±2)℃的冰箱冷藏室,可有效保存240天以上,在MS+琼脂7 g/L+蔗糖50 g/L的培养基上,试管苗的生长得到明显的抑制。保存后的试管苗增殖、生根、驯化效果良好,生长正常。
Propagation system of vitro buds were effectively established from young stem segments of Sorbus sibirica being sterilized with 0.1% mercuricchloride for 6 minutes and cultured in MS propagation medium （MS＋6BA0.8 mg/L＋NAA0.08 mg/L＋KT0.4 mg/L＋ IBA0.04 mg/L）,different conservation conditions and mediums were studied on conservation effects of in vitro plant.The results show that vitro plants could be effectively conserved in refrigerator（2±2）℃more than 8 months,and their growth were significantly inhibited in medium（MS＋agar 7 g/L＋ sucrose 50 g/L） and the in vitro conserved seedlings propagated,rooted and acclimatized and grew well.
出处
《林业科技开发》
2011年第1期100-102,共3页
China Forestry Science and Technology
基金
沈阳市科技局计划项目:"西伯利亚花楸等珍贵观赏树种在城乡景观功能区建设中的应用与示范"(编号:1071159-3-00)
关键词
西伯利亚花楸
试管苗
保存
再培养
Sorbus sibirica
in vitro plant
conservation
subculture
