克氏原螯虾的血淋巴细胞原代培养

The Primary Cell Culture of Crawfish Hemocytes

取克氏原螯虾（ Ｐｒｏｃａｍｂａｒｕｓ ｃｌａｒｋｉｉ） 血淋巴液， 与抗凝剂混匀后离心， 去上清， 用含１５ ％ 犊牛血清及１００ Ｕ／ｍＬ青霉素、１００ μｇ／ｍＬ 链霉素的Ｌ１５ 培养液悬浮血淋巴细胞， 移至培养瓶， （２８ ±１） ℃培养。细胞于１２ ｈ 内贴壁， 形态不一， 可见透明细胞、小颗粒细胞及大颗粒细胞３ 种类型， 多数不分裂， 可维持１５ ｄ 以上。上述方法重复至少２５ 次，

Hemocytes of crawfish(Procambarus clarkii)were attached to the flask within 12 h at (28±1)℃,supported by L 15 medium containing 15% calf serum,Penicilin 100 U/mL and Streptomycin 100 μg/mL.Cells could be maintained for 2 weeks at least,most of which were not in an active state of division.The structure of cells varied and could be classified into three types:hyaline,semigranular and granular cells.