反相高效液相色谱法测定磷酸川芎嗪片的含量

Content Determination of Ligustrazine Phosphate Tablets by RP-HPLC

目的建立测定磷酸川芎嗪片含量的反相高效液相色谱法。方法采用XDB-C18分析柱(150.0 mm×4.6 mm,6μm),流动相为甲醇-1%醋酸溶液(35∶65),流速1.0mL/min,检测波长295nm;并用紫外分光光度法做对比试验。结果磷酸川芎嗪的保留时间为6.82min,其质量浓度在5～40μg/mL范围内与峰面积线性关系良好(r=0.9998,n=6),平均回收率为99.02%,RSD为0.46%(n=6)。结论所用方法简单、准确,重复性好,可作为磷酸川芎嗪片的含量测定方法。

Objective To set up an RP-HPLC method for the content determination of Ligustrazine Phosphate Tablets.Methods The XDB-C18 column（150.0 mm×4.6 mm,6 μm） was used as the separation column.The mobile phase was methanol-1% acetic acid（35 ∶65）.The flow rate was 1.0 mL/min,and the detection wavelength was 295 nm.The comparison test was carried out with ultra-violet spectrophotometry.Results The retention time of ligustrazine phosphate was 6.82 min.The good linear relationship was shown within the range of 5-40 μg/mL（r=0.999 8,n=6） for the concentrations of ligustrazine phosphate.The average recovery rate was 99.02%,RSD=0.46%（n=6）.Conclusion The method is convenient,rapid,accurate and well reproducible,which may be used for the content determination of Ligustrazine Phosphate Tablets.