MMP-2、TIMP-2和NF-κB p^(65)在甲状腺乳头状癌中的表达及意义

Expression and Significance of MMP-2,TIMP-2 and NF-κB p^(65) in Papillary Thyroid Carcinoma

目的探讨MMP-2、TIMP-2及NF-κB在甲状腺乳头状癌中的表达及临床意义。方法采用免疫组化和原位杂交方法检测157例甲状腺乳头状癌、30例甲状腺乳头状瘤和30例正常甲状腺组织中MMP-2、TIMP-2及NF-κB的表达,并用χ2检验进行统计学处理。结果 MMP-2及NF-κB的表达甲状腺乳头状癌和甲状腺乳头状瘤明显高于正常甲状腺组织(P<0.01);并且在甲状腺乳头状癌中的表达随着病理分级增高而显著增加(P<0.01);甲状腺乳头状癌患者有颈部淋巴结转移者明显高于无转移者(P<0.01)呈正相关。TIMP-2阳性表达正常甲状腺组织明显高于甲状腺乳头状癌和甲状腺乳头状瘤(P<0.05);并随着恶性肿瘤分级增高而显著降低(P<0.05);有颈淋巴结转移低于无转移者(P<0.01)呈负相关。结论 MMP-2、TIMP-2及NF-κB在甲状腺良、恶肿瘤和正常甲状腺组织中不同程度的异常表达,它们均可作为判断甲状腺肿瘤生物学行为及患者预后参考指标。它们可能有协同致瘤及恶性肿瘤侵袭和转移作用,具有重要的临床应用价值。

Objective To investigate the expression and clinical significance of MMP-2, TIMP-2 and NF-κB p65 in papillary thyroid carcinoma. Methods The expression of MMP-2 ,TIMP-2 and NF-κB in 157 cases of papillary thyroid cancer and 30 cases of papillary thyroid tumors and 30 cases of thyroid tissue were detected by immunohistochemistry and in situ hybridization, and the data were analyzed by x2 test. Results The expression of MMP-2 protease and NF-κB p65 protein and mRNA in papillary thyroid cancer and benign tumors was significantly higher than that of thyroid tissue（ P 〈 0.01 ） , And in papillary thyroid carcino- ma with the tumor grade increased significantly increased（ P 〈 0. 01 ） ;there was a positive correlation. And the expression in cervical lymph node metastasis was higher than those without metastasis （P 〈 0. 01 ）. And the expression of TIMP-2 protein and mRNA was contrast with MMP-2, there was a negative correlation. Conclusion There were different degrees of abnormal expression of MMP-2 ,TIMP-2 and NF-κB p65 protein and mRNA in thyroid benign and malignant tumors and normal thyroid tissue,they can be used as markers to determine the biological behavior of thyroid tumors and the prognosis of patients. They may have synergistic effects and relation with tumor invasion and metastasis. They have important clinical value.