IKCal蛋白产物在人宫颈鳞状上皮细胞癌组织及正常宫颈组织中表达差异分析

The expression of intermediate-conductance-Ca^(2+)-activated K^+ channels′protein in the tissues of cervical cancer

目的研究人宫颈鳞状上皮细胞癌组织及正常宫颈上皮组织中钙激活性中电导钾离子通道(IKCal)蛋白产物的分布差异,探讨IKCal与人类宫颈鳞状上皮细胞癌的关联。方法采用免疫组织化学方法检测18例正常宫颈上皮组织和30例不同分化程度的宫颈鳞状上皮细胞癌组织中IKCal蛋白产物表达部位及表达水平。结果①IKCal在30例宫颈鳞状上皮细胞癌组织细胞核及细胞膜上均呈阳性表达;18例正常宫颈上皮组织中仅1例在鳞状上皮部位细胞膜部位有较弱表达,IKCal蛋白产物在宫颈鳞癌组织中的表达与正常宫颈组织中存在明显差异(Z=-6.109,P=0.00,P<0.05);②在不同分化程度的宫颈鳞癌组织中,IKCal蛋白产物表达程度与癌细胞分化程度呈负相关(2χ=16.468,P=0.00,P<0.017)。结论宫颈鳞癌组织中IKCal蛋白产物的表达明显高于正常宫颈组织;且IKCal蛋白产物表达水平与癌细胞的分化程度呈负相关;过高表达的IKCal与宫颈鳞状上皮细胞癌的发生和发展可能存在重要联系。

Objective To compare the intermediate-conductance-Ca2＋-activated K＋channels（IKCal） protein in the tissues of cervical cancer with the normal tissue by the immuno-histochemical method.Methods The immuno-histochemical method was used to examine the IKCal protein in the 18 cases normal cervical tissue and 30 cases of different differentiation of cancer cells.Results ①All the specimens of cervical cancer were expressing the IKCal protein in the nucletis;there was only 1 case expressing the IKCal antibody.The expression rate of IKCal protein in cervical cancer was higher than in normal cervix（Z=-6.109,P=0.00,P0.05）.②the expressions of IKCal protein in the cervical cancer were related in the degrees of differentiation of the cancer cells（χ2=16.468,P=0.00,P0.017）.Conclusion There are higher expressions of IKCal protein in the cervical cancer than in the normal cervix.The relationship between the degrees of differentiation of cervical cancer＇ cells and the expressions of IKCal protein is negative correlation.The over-expression of IKCal should be related with the occurrence and development of cervical cancer.