摘要
RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40,respectively. In this study,we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.
RNA interference （RNAi） is a process by which introduced small interfering RNA （siRNA） can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 （HSV-1） RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40,respectively. In this study,we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.
基金
The Nation "863" Program of China(2006AA02A226)
The Joint Funds of National Science Foundation of China (U0632010)
The State KeyLaboratory of Phytochemistry and Plant Resources in West China
Chinese Academy of Sciences (O807B11211, O807E21211)
"211 grant of MOE"
