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拮抗酵母菌0732-1的鉴定及其ITS-5.8S rDNA序列分析 被引量:1

Identification and ITS-5.8S rDNA Sequence Analysis of the Antagonistic Yeast Strain 0732-1 for Against Acidovorax avena subsp.citrulli
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摘要 从石河子地区西瓜叶片上分离获得1株对哈密瓜细菌性果斑病菌-燕麦嗜酸菌属西瓜亚种(Acidovorax avena subsp.citrulli)有显著拮抗作用的酵母菌0732-1,对该菌株形态学观察、培养性状观察、生理生化测定,以及结合ITS-5.8S rDNA序列同源性分析。结果表明:酵母菌0732-1为异常毕赤酵母(Pichiaanomala Kurtzman),首次证明了P.anomala对哈密瓜细菌性果斑病菌具有生防作用。 Yeast strain 0732-1,isolated from watermelon leaf samples of Tianshan reservation region,showed inhibitory activity when its filtrate was drop onto the KBA medium plate surface spreading evenly A.avena subsp citrull i.Based on its morphological,cultural characteristics,general physiological and biochemical properties and analysis of the DNA sequence of the internal transcribed spacer of ribosomal DNA(The genbank accession numbe EU380207),the yeast strain 0732-1 was identified as Pichia anomala Kurtzman.To our knowledge,this is the first report of P.anomala with potential for biocontrol of bacterial fruit blotch of hami melon caused by A.avena subsp.citrulli.
出处 《北方园艺》 CAS 北大核心 2011年第1期146-149,共4页 Northern Horticulture
基金 石河子大学高层次人才科研启动资助项目(RCZX200906)
关键词 细菌性果斑病 酵母菌 ITS-5.8S RDNA 种类鉴定 bacterial fruit blotch yeast ITS-5.8S rDNA identification
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