荷斯坦牛羊水来源干细胞分离培养及其表面标志检测

Isolation of amniotic fluid-derived stem cells from Holstein cow and test of the cell markers

利用羊水来源干细胞培养技术体系,从胎龄285 d荷斯坦奶牛胎儿羊水中分离培养干细胞;采用RT-PCR技术检测干细胞表面标志或相关基因。成功分离培养出奶牛羊水来源干细胞,干细胞中CD-90、Nanog、Oct4、TERT、Sox2、Desmin和HES1表达阳性。从奶牛胎儿羊水中分离干细胞具有可行性和有效性,为进行转基因研究提供靶细胞奠定了基础。

The aim of this study was to get amniotic fluid-derived stem（AFS） cells from cow which may be used as target cells for transgenic research in future.AFS cells were isolated from amniotic fluid of a 285-day-old Holstein cow fetus.Expanded AFS cells were harvested for analysis of markers using reverse transcription-polymerase chain reaction（RT-PCR）.The results showed that AFS cells were abtained from amniotic fluid.AFS cells expressed CD-90,Nanog,Oct4,TERT,Sox2,Desmin and HES1.We have demonstrated that stem cells isolated from amniotic fluid of cow may be used as target cells for transgenic research in future.