摘要
细菌的致病过程涉及多个毒力基因表达产物的相互作用。然而,随着越来越多的微生物基因组测序工作的相继或即将完成,对其毒力基因的研究却相对滞后。这不但影响了致病机理等基础研究的进展,还成为制约抗菌药物和疫苗研发的瓶颈。本文从生物信息学、筛选差异表达基因等4个方面介绍了筛选细菌毒力基因的方法及其应用,并综合了研究致病菌与宿主间相互作用关系的方法。为发现和研究致病菌新的毒力因子,理顺病原与宿主关系开拓了思路。
The research indicated that bacterial pathogenesis involves the interaction of several virulence genes.However,as an increasing number of microbial genome sequencing,the study of its virulence genes is lagging. Not only this affect pathogenesis and other basic research,but also restrict the development of antibiotics and vaccine.This article introduces the methods of screening bacterial virulence genes and its application in four aspects such as bioinformatics and screening genes of differential expressed,and also includes the methods to study the interaction between pathogen and host.The purpose is to develop ideas for discovering and studying new virulence factors of pathogen,and find out the relation between pathogen and host.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2011年第2期288-291,共4页
Chinese Journal of Veterinary Science
基金
国家重点基础研究发展计划(973)项目(2006CB504-403)
国家"十一五"科技支撑计划项目(2006BAD06A01
2006BAD06A12)
关键词
细菌
毒力基因
筛选方法
应用
bacteria
virulence gene
methods of screening
application
